Directing cell migration using micropatterned and dynamically adhesive polymer brushes |
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| Affiliation: | 1. Barts and the London School of Medicine and Dentistry, Queen Mary, University of London, 4 Newark Street, London E1 2AT, UK;2. School of Engineering and Materials Science, Queen Mary, University of London, Mile End Road, London E1 4NS, UK;3. Institute of Bioengineering, Queen Mary, University of London, London, UK;1. Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands;2. Department of Materials Science and Technology of Polymers, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands;3. Laboratory for Surface Science and Technology, Department of Materials, ETH Zurich, Vladimir-Prelog-Weg 5, 8093 Zurich, Switzerland;4. Department of Complex Tissue Regeneration, Faculty of Health, Medicine and Life Sciences, Maastricht University, P.O. Box 616, 6200MD Maastricht, The Netherlands;1. State-key Laboratory for Mechanical Behavior of Materials, Xi''an Jiaotong University, Xi''an 710049, China;2. Department of Osteology, Xi''an People’s Hospital (Xi''an No. 4 Hospital), Xi''an 710100, China;3. Department of Osteology, Tangdu Hospital, Fourth Military Medical University, Xi''an 710038, China;1. Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Science, Ningbo 315201, China;2. State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;3. Institute of Printing and Packaging Engineering, Xi’an University of Technology, Xi’an 710048, China;1. Chair of Chemistry of Polymeric Materials & Christian Doppler Laboratory for Functional and Polymer Based Ink-Jet Inks, University of Leoben, Otto-Glöckel-Strasse 2, A-8700 Leoben, Austria;2. Durst Phototechnik DIT, Julius-Durst-Strasse 11, A-9900 Lienz, Austria |
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| Abstract: | Micropatterning techniques, such as photolithography and microcontact printing, provide robust tools for controlling the adhesive interactions between cells and their extracellular environment. However, the ability to modify these interactions in real time and examine dynamic cellular responses remains a significant challenge. Here we describe a novel strategy to create dynamically adhesive, micropatterned substrates, which afford precise control of cell adhesion and migration over both space and time. Specific functionalization of micropatterned poly(ethylene glycol methacrylate) (POEGMA) brushes with synthetic peptides, containing the integrin-binding arginine–glycine–aspartic acid (RGD) motif, was achieved using thiol–yne coupling reactions. RGD activation of POEGMA brushes promoted fibroblast adhesion, spreading and migration into previously non-adhesive areas, and migration speed could be tuned by adjusting the surface ligand density. We propose that this technique is a robust strategy for creating dynamically adhesive biomaterial surfaces and a useful assay for studying cell migration. |
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| Keywords: | Polymer brush Micropattern Click chemistry Cell migration Integrin |
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