Photobleaching as a tool to measure the local strain field in fibrous membranes of connective tissues |
| |
| Affiliation: | 1. Université de Lyon, F-69622 Lyon;IFSTTAR, LBMC, UMR-T9406; Université Lyon 1, France;2. Plateforme IVTV, CNRS, 36 Avenue Guy de Collongue, Bâtiment G8, 69134 Ecully Cedex, France;3. LUNAM Université, GEM, UMR CNRS 6183, Ecole Centrale de Nantes, Université de Nantes, France;1. Department of Biomedical Engineering and Physics, Academic Medical Centre, Meibergdreef 9, 1105 AZ Amsterdam, the Netherlands;2. Department of Molecular Biophysics, Utrecht University, 3508 TA Utrecht, The Netherlands;3. Centre for Optical Diagnostics and Therapy, Erasmus Medical Centre, Post Box 2040, 3000 CA, Rotterdam, the Netherlands;1. The School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA;2. The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA;3. The Parker H. Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA 30332, USA;1. JSOL Corporation, 2-2-4, Tosabori, Ninshi-ku, Osaka, 550-0001, Japan;2. Honda R&D Co.,Ltd., 4630, Shimotakanezawa,Haga-machi,Haga-gun, Tochigi, 321-3393, Japan;3. JFE Steel Corporation, 1, Kawasaki-cho, Chuoku, Chiba, 260-0835, Japan;1. Department of Bioengineering, University of Pennsylvania, 240 Skirkanich Hall, 210 South 33rd St, Philadelphia, PA 19104, USA;2. Department of Biomedical Engineering, University of Delaware, 125 East Delaware Avenue, Newark, DE 19716, USA;1. Université Paris-Est, Laboratoire Modélisation et Simulation Multi-Échelle, MSME UMR 8208 CNRS, 61, Av. du Général de Gaulle, 94010 Créteil, France;2. Université Paris-Est, Laboratoire Modélisation et Simulation Multi-Échelle, MSME UMR 8208 CNRS, 5, bd Descartes, 77454 Marne-la-Vallée, France |
| |
| Abstract: | Connective tissues are complex structures which contain collagen and elastin fibers. These fiber-based structures have a great influence on material mechanical properties and need to be studied at the microscopic scale. Several microscopy techniques have been developed in order to image such microstructures; among them are two-photon excited fluorescence microscopy and second harmonic generation. These observations have been coupled with mechanical characterization to link microstructural kinematics to macroscopic material parameter evolution. In this study, we present a new approach to measure local strain in soft biological tissues using a side-effect of fluorescence microscopy: photobleaching. Controlling the loss of fluorescence induced by photobleaching, we create a pattern on our sample that we can monitor during mechanical loading. The image analysis allows three-dimensional displacements of the patterns at various loading levels to be computed. Then, local strain distribution is derived using the finite element discretization on a four-node element mesh created from our photobleached pattern. Photobleaching tests on a human liver capsule have revealed that this technique is non-destructive and does not have any impact on mechanical properties. This method is likely to have other applications in biological material studies, considering that all collagen–elastin fiber-based biological tissues possess autofluorescence properties and thus can be photobleached. |
| |
| Keywords: | Photobleaching Strain measurement Multiphoton microscopy Collagen Elastin |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
