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Banded structures in collagen vitrigels for corneal injury repair
Affiliation:1. The Johns Hopkins University, Applied Physics Laboratory, Laurel, MD 20723, USA;2. The Wilmer Eye Institute, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA;3. State University of New York at Stony Brook, Stony Brook, NY 11794-3400, USA;4. The Johns Hopkins University, Department of Biomedical Engineering, Baltimore, MD 21231, USA;1. Department of Materials Sciences and Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan;2. Advanced Delivery Technology Co. Ltd, 5F, D Building, No. 120, Zhonghua Rd, Hsinchu Industrial Park, Hukou Township, Hsinchu 30352, Taiwan;1. Department of Ophthalmology, Jacobs Retina Center/Shiley Eye Center, University of California, San Diego, La Jolla, CA, USA;2. Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, People’s Republic of China;3. Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, USA;1. MedShape, Inc., 1575 Northside Drive NW Suite 440, Atlanta, GA 30318, USA;2. School of Materials Science and Engineering, Georgia Institute of Technology, 771 Ferst Drive, Atlanta, GA 30332, USA;3. Department of Medicine, Division of Cardiology, Emory University School of Medicine, 1639 Pierce Drive, Atlanta, GA 30322, USA;4. Atlanta Veterans Affairs Medical Center, 1670 Clairmont Rd, Decatur, GA 30033, USA;5. W.H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 313 Ferst Drive, Atlanta, GA 30332, USA;6. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 801 Ferst Drive, Atlanta, GA 30332, USA;1. Department of Orthopedic Surgery, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA;2. Institute for Regenerative Engineering, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA;3. Center for Molecular Medicine, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA;4. Department of Materials Science and Engineering and Department of Biomedical Engineering, University of Connecticut Storrs, CT 06268, USA;1. Department of Orthopaedic Surgery, Stanford University School of Medicine, 450 Broadway Street, Redwood City, CA 94063, USA;2. Department of Bioengineering, Stanford University, Stanford, CA, USA;3. Department of Cardiovascular Research, Development, and Translational Medicine, Kyushu University, Japan;1. Molecular Immunology Unit, Institute of Child Health, University College London, London, United Kingdom;2. Great Ormond Street Hospital National Health Service Trust, London, United Kingdom;3. London Centre for Nanotechnology and Department of Physics and Astronomy, University College London, London, United Kingdom;4. Neural Development Unit, Institute of Child Health, University College London, London, United Kingdom;5. London Centre for Nanotechnology and Department of Cell & Developmental Biology, University College London, London, United Kingdom;6. Peninsula College of Medicine and Dentistry, Peninsula Medical School, University of Exeter, St Luke''s Campus, Exeter, United Kingdom
Abstract:There is a growing interest in using collagen vitrigels for corneal injury repair. We recently reported the synthesis and thermal denaturation behavior of these gels. In this paper, the banded structure in these vitrified gels is studied by small-angle X-ray scattering (SAXS) one-dimensional (1-D) correlation function analysis and transmission electron microscopy (TEM). Results demonstrate that the collagen vitrigel possess banded structures similar to those of the starting type I collagen, with an average D-spacing of 64 nm (by SAXS) or 57 nm (by TEM). A combination of SAXS 1-D correlation function analyses and TEM show that overlap and gap distances ranged from 30 to 33 nm and from 23 to 25 nm, respectively. Changing the vitrification condition does not impact on the banded structure significantly.
Keywords:collagen vitrigel  Corneal injury repair  Banded structure  Small-angle X-ray scattering
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