急性心肌梗死病人血清微RNA-1283表达与中医证型的关系分析

目的 探讨急性心肌梗死病人血清微RNA-1283(miR-1283)表达与中医证型的关系。方法 选择北京市怀柔区中医医院2018年12月至2021年11月收治的236例急性心肌梗死病人作为疾病组，另选取同期在该院体检的健康人群230例作为健康组。采用实时荧光定量聚合酶链式反应PCR(RT-qPCR)法检测两组病人血清miR-1283表达情况并对比。另根据中医辨证分型标准将疾病组分为不同证型，比较疾病组不同中医证型病人血清miR-1283表达水平。结果 疾病组miR-1283表达水平（0.42±0.07）低于健康组（0.98±0.18）(t=44.47,P<0.001)；疾病组236例病人经中医辨证分型，分为实证99例、虚实夹杂证111例、虚证26例，其中痰瘀互结型、寒凝心脉型、气虚血瘀型、气虚痰瘀互阻型、气虚痰浊闭阻型、气阴两虚型、阴竭阳脱型占比分别为38.98%(92/236)、2.97%(7/236)、27.12%(64/236)、11.86%(28/236)、8.05%(19/236)、8.90%(21/236)、2.12%(5/236)。寒凝心脉型、气虚血瘀型、气虚痰瘀互...

Analysis of the relationship between serum microRNA-1283 expression and TCM syndrome types in patients with acute myocardial infarction

Objective To explore the relationship between the expression of serum microRNA-1283 (miR-1283) and Traditional Chinese Medical (TCM) syndrome types in patients with acute myocardial infarction.Methods A total of 236 patients with acute myocardial infarction admitted to Huairou District Traditional Chinese Medicine Hospital in Beijing from December 2018 to November2021 were selected as the disease group, and 230 healthy people who underwent physical examination in Huairou District Hospital ofTraditional Chinese Medicine in the same period were selected as the health group. The expressions of serum miR-1283 in the two groups were detected by real-time fluorescence quantitative polymerase chain reaction PCR (RT-qPCR) and compared. In addition, thedisease groups were divided into different syndrome types according to the dialectical typing standard of TCM, and the expression levels of serum miR-1283 in patients with different TCM syndrome types in the disease groups were compared.Results The expression level of miR-1283 in the disease group (0.42±0.07) was lower than that in the healthy group (0.98±0.18) (t=44.47, P<0.001). Accordingto TCM syndrome differentiation, 236 patients in the disease group were divided into 99 cases of excess syndrome, 111 cases of deficiency and excess syndrome and 26 cases of deficiency syndrome, and the proportions of phlegm and blood stasis type, cold coagulationheart pulse type, qi deficiency and blood stasis type, qi deficiency and phlegm stasis mutual resistance type, qi deficiency and phlegmturbidity closed resistance type, qi yin deficiency type and yin exhaustion and yang removal type were respectively 38.98,% (92/236),2.97% (7/236), 27.12% (64/236), 11.86% (28/236), 8.05% (19/236), 8.90% (21/236), 2.12% (5/236). There were no significant differences in the expression levels of serum miR-1283 in patients with cold coagulation heart pulse type, qi deficiency and blood stasis type,qi deficiency and phlegm stasis mutual resistance type, qi deficiency and phlegm turbidity closed resistance type and qi yin deficiencytype in pairwise comparison (P>0.05). The expression level of serum miR-1283 in patients with yin exhaustion and yang removal typewas higher than that in phlegm and blood stasis type, cold coagulation heart pulse type, qi deficiency and blood stasis type, qi deficiency and phlegm stasis mutual resistance type, qi deficiency and phlegm turbidity closed resistance type and qi yin deficiency type (P< 0.05). The expression level of serum miR-1283 in patients with phlegm and blood stasis type was lower than that in patients with coldcoagulation heart pulse type, qi deficiency and blood stasis type, qi deficiency and phlegm stasis mutual resistance type, qi deficiencyand phlegm turbidity closed resistance type and qi Yin deficiency type (P<0.05). Conclusion The expression level of serum miR1283 in patients with acute myocardial infarction is low, and there are differences in the expression level of serum miR-1283 in patientswith different syndrome types, suggesting that this index can be used as the basis of TCM syndrome classification in patients with acutemyocardial infarction.