Macrophage mannose receptor-specific gene delivery vehicle for macrophage engineering |
| |
Affiliation: | 1. Division of Research Instrument and Equipment, Life Science Research Center, Kagawa University, Kagawa, Japan;2. Department of Endocrinology, Faculty of Medicine, Kagawa University, Kagawa, Japan;3. Department of Bacteriology, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, Okayama, Japan;1. Laboratory of Pharmaceutical Engineering, EA 4267, University of Franche-Comté, Besançon, France;2. Department of Pharmaceutics, Institute of Pharmacy, University of Bonn, Germany;1. Department of Industrial and Physical Pharmacy, Purdue University, 575 Stadium Mall Drive, West Lafayette, IN 47907, United States;2. Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, United States |
| |
Abstract: | Macrophages are the most plastic cells in the hematopoietic system and they exhibit great functional diversity. They have been extensively applied in anti-inflammatory, anti-fibrotic and anti-cancer therapies. However, the application of macrophages is limited by the efficiency of their engineering. The macrophage mannose receptor (MMR, CD206), a C-type lectin receptor, is ubiquitously expressed on macrophages and has a high affinity for mannose oligosaccharides. In the present study, we developed a novel non-viral vehicle with specific affinity for MMR. Mannan was cationized with spermine at a grafted ratio of ∼12% to deliver DNA and was characterized as a stable system for delivery. This spermine–mannan (SM)-based delivery system was evaluated as a biocompatible vehicle with superior transfection efficiency on murine macrophages, up to 28.5-fold higher than spermine–pullulan, 11.5-fold higher than polyethylenimine and 3.0-fold higher than Lipofectamine™ 2000. We confirmed that the SM-based delivery system for macrophages transfection was MMR-specific and we described the intracellular transport of the delivery system. To our knowledge, this is the first study using SM to demonstrate a mannose receptor-specific gene delivery system, thereby highlighting the potential of a novel specific non-viral delivery vehicle for macrophage engineering. |
| |
Keywords: | Gene delivery Mannose receptor Macrophage Spermine–mannan |
本文献已被 ScienceDirect 等数据库收录! |
|