Topologically defined composites of collagen types I and V as in vitro cell culture scaffolds |
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| Affiliation: | 1. Biophysical Chemistry Group, Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, Universität Leipzig, Leipzig 04103, Germany;2. Department of Dermatology, Venerology and Allergology, Universität Leipzig, Leipzig 04103, Germany;1. 3B’s Research Group—Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence of Tissue Engineering and Regenerative Medicine, AvePark, Zona Industrial da Gandra, S. Cláudio do Barco, 4806-909 Caldas das Taipas—Guimarães, Portugal;2. ICVS/3B’s, PT Government Associated Laboratory, Braga/Guimarães, Portugal;3. G.I.R. Bioforge, University of Valladolid, Edificio I+D, Paseo de Belén, 11, 47011 Valladolid, Spain;4. Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Valladolid, Spain;3. Institute of Biostructures and Bioimaging, Consiglio Nazionale delle Ricerche, Via Mezzocannone 16, I-80134 Napoli, Italy;4. Department of Microbiology, Immunology, and Cell Biology, West Virginia University School of Medicine, Morgantown, West Virginia 26506;5. Division of Molecular Biosciences, Imperial College London, London SW7 2AZ, United Kingdom;1. Department of Chemistry, Stanford University, Stanford, CA 94305, United States;2. Department of Mechanical Engineering, Stanford University, 452 Escondido Mall, Stanford, CA 94305, United States;3. Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305, United States;1. Theodor Boveri Institute and Comprehensive Cancer Center Mainfranken, Biocenter, University of Wuerzburg, Wuerzburg 97074, Germany;2. Comprehensive Cancer Center Mainfranken, Core Unit Bioinformatics, Biocenter, University of Wuerzburg, Wuerzburg 97074, Germany;3. Cancer Systems Biology Group, Biochemistry and Molecular Biology, University of Wuerzburg, Wuerzburg 97074, Germany;4. Institute of Pathology and Comprehensive Cancer Center Mainfranken, University of Wuerzburg, Wuerzburg 97080, Germany;5. Leibniz Institute on Aging, Fritz Lipmann Institute e.V., Jena 07745, Germany |
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| Abstract: | Cell fate is known to be triggered by cues from the extracellular matrix, including its chemical, biological and physical characteristics. Specifically, mechanical and topological properties are increasingly recognized as important signals. The aim of this work was to provide an easily accessible biomimetic in vitro platform of topologically defined collagen I matrices to dissect cell behaviour under various conditions in vitro. We reconstituted covalently bound layers of three-dimensional (3-D) networks of collagen type I and collagen type V with a defined network topology. A new erosion algorithm enabled us to analyse the mean pore diameter and fibril content, while the mean fibril diameter was examined by an autocorrelation method. Different concentrations and ratios of collagen I and V resulted in pore diameters from 2.4 to 4.5 μm and fibril diameters from 0.6 to 0.8 μm. A comparison of telopeptide intact collagen I to telopeptide deficient collagen I revealed obvious differences in network structure. The good correlation of the topological data to measurements of network stiffness as well as invasion of human dermal fibroblasts proves that the topological analysis provides meaningful measures of the functional characteristics of the reconstituted 3-D collagen matrices. |
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| Keywords: | Collagen structure Topology Image analysis Mechanical properties Fibroblast |
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