Development of an alternative non-obese non-genetic rat model of type 2 diabetes using caffeine and streptozotocin |
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| Institution: | 1. Departamento de Patologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, Brazil;2. Department of Chemistry and Biochemistry, University of Arizona, Tucson, USA;3. Department of Pharmacology, Ribeirão Preto Medical School, University of São Paulo, São Paulo, Brazil;4. Department of Pharmaceutical Sciences, University Hospital (Health Science Centre), Londrina State University, Parana, Brazil;1. Department of Pharmacological Screening, Chair of Pharmacodynamics, Jagiellonian University, Medical College, Krakow, Poland;2. Chair of Pharmacobiology, Jagiellonian University, Medical College, Krakow, Poland;3. Chair of Pharmaceutical Chemistry, Department of Physicochemical Drug Analysis, Jagiellonian University, Medical College, Krakow, Poland;4. Chair of Pharmacodynamics, Jagiellonian University, Medical College, Krakow, Poland;5. Laboratory of Trace Elements Neurobiology, Department of Neurobiology, Institute of Pharmacology, Polish Academy of Sciences and Center of Excellence in Neuropsychopharmacology, Krakow, Poland;1. University Institute of Pharmaceutical Sciences, UGC Center of Advanced Study (UGC-CAS) in Pharmaceutical Sciences, Panjab University, Chandigarh, India;2. School of Pharmacy, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago;3. Swift School of Pharmacy, Swift Group of Colleges, Rajpura, India;4. Department of Chemistry, UGC Center of Advanced Study (UGC-CAS) in Chemistry, Panjab University, Chandigarh, India;1. Department of Pharmacogenetics, Medical University of Lodz, ?ód?, Poland;2. Department of General and Colorectal Surgery, Medical University of Lodz, ?ód?, Poland |
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| Abstract: | BackgroundThe aim of the present study was to develop an alternative non-obese non-genetic rat model of type 2 diabetes (T2D).MethodsSix-week-old male SD rats were randomly divided into six groups, namely: Normal Control (NC), Diabetic Control (DBC), Caffeine 5 mg/kg BW + STZ (CAF5), Caffeine 10 mg/kg BW + STZ (CAF10), Caffeine 20 mg/kg BW + STZ (CAF20) and Caffeine 40 mg/kg BW + STZ (CAF40) and were fed a normal rat pellet diet and drinking water ad libitum throughout the experimental period. After a one week acclimatization period, diabetes was induced in the animals in DBC and all CAF groups with an injection (i.p.) of the respective dosages of caffeine (mg/kg BW) 15 min before the injection of STZ (65 mg/kg BW) when normal saline was injected to the DBC group instead of caffeine. The NC group received normal saline and buffer instead of caffeine and STZ, respectively. One week after the STZ injection, animals with non-fasting blood glucose > 300 mg/dl were considered as diabetic. Three weeks after the STZ injection, the animals in the CAF5 and CAF10 groups were eliminated from the study due to the severity of diabetes and the experiment was continued with the remainder groups for a 13 weeks period.Results and conclusionThe data of food and fluid intake, body weight, blood glucose, glucose tolerance test, HOMA-IR, HOMA-beta, serum insulin, fructosamine, lipid profile and organ specific enzymes, anti-diabetic drug response tests, and pancreatic histopathology suggest that CAF20 group can be a better alternative non-genetic model of non-obese T2D. |
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| Keywords: | Caffeine Streptozotocin Non-obese Type 2 diabetes Rat model |
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