中药金贯蓝提取物对寻常痤疮相关致病菌的体外抑菌作用

目的 考察自主研发的协定方中药“金贯蓝”提取物对寻常痤疮三种主要致病菌（痤疮丙酸杆菌、表皮葡萄球菌、金黄色葡萄球菌）的体外抑菌效果，为其临床治疗寻常痤疮提供一定实验依据。方法 2021年1月至2022年3月采用琼脂打孔法考察金贯蓝提取物对三种痤疮相关致病菌的抑菌活性，并评估不同浓度药物对上述菌的抗菌作用是否具有浓度相关性；采用倍比稀释法测定药物最低抑菌浓度以及最小杀菌浓度，绘制药物时间抑菌曲线进一步考察其作用特点。结果 500 g/L金贯蓝提取物对C. acnes、S. epidermidis、S. aureus的抑菌圈直径分别为（27.67±2.52）mm、（23.33±1.53）mm、（22.00±1.00）mm；浓度为250 g/L金贯蓝提取物对C. acnes、S. epidermidis、S. aureus的抑菌圈直径分别为（22.33±0.58）mm、（20.67±1.53）mm、（20.11±1.20）mm；浓度为100 g/L金贯蓝提取物对C. acnes、S. epidermidis、S. aureus的抑菌圈直径分别为（13.30±0.75）mm、（10.67±1....

In vitro antibacterial effect of Chinese herbal extracts of Jinguanlan on acne vulgaris-related pathogens

Objective To investigate the effect of Jinguanlan extract on the three main pathogenic bacteria of acne vulgaris (Cutibac? terium acnes, Staphylococcus epidermidis and Staphylococcus aureus), so as to provide a certain experimental basis for its clinical treatment of acne vulgaris.Methods From January 2021 to March 2022, Agar drilling method was used to investigate the antibacterial activity of the extract against three acne-related pathogenic bacteria, and to evaluate the concentration correlation of the antibacterial effect ofdifferent concentrations of drugs on the above-mentioned bacteria. The minimum inhibitory concentration and minimum bactericidalconcentration were determined by multiple dilution method, and the time inhibition curve was drawn to further investigate its action characteristics.Results The bacteriostatic zone diameters of 500 g/L Jinguanlan extract against C. acnes, S. epidermidis and S. aureus were (27.67±2.52) mm, (23.33 ±1.53) mm and (22.00±1.00) mm, respectively. The bacteriostatic zone diameters of 250 g/L Jinguanlan extract against C. acnes, S. epidermidis and S. aureus were (22.33 ±0.58) mm, (20.67 ±1.53) mm and (20.11 ±1.20) mm, respectively. Thebacteriostatic zone diameter of 100 g/L the extract against C. acnes, S. epidermidis and S. aureus were (13.30±0.75) mm, (10.67±1.53)mm and (10.67±0.58) mm, respectively. There was a positive correlation between the diameter of the antibacterial zone and the concentration of the extract (r>0.90 P<0.001). The MIC of Cutibacterium acnes, Staphylococcus epidermidis, and Staphylococcus aureus were all 25 g/L; MBC was 50 g/L, 50 g/L, 25 g/L, respectively. The bacteriostatic curve showed that it mainly affected the slow period of bacterialgrowth.Conclusion The extract of JINGUANLAN formula has a good inhibitory effect on the three main pathogenic bacteria of acnevulgaris, and it is positively correlated with the drug concentration, which is worthy of further development and research.