circ_0009910在胃癌细胞中作用机制初步研究

目的通过检测分析胃癌细胞中circ_0009910表达,初步探讨其在胃癌细胞中的作用机制。方法用qRT-PCR检测胃癌细胞系BGC823、SGC7901、AGS、MGC803、MKN45中的circ_0009910表达水平,在BGC823、AGS细胞中采用circ_0009910 siRNA转染敲降circ_0009910,验证转染效果;在circ_0009910敲降的BGC823、AGS细胞中,用MTT法检测细胞增殖、平板法检测集落形成、Transwell检测迁移和侵袭、western-blotting检测上皮间质转化(EMT),采用SPSS 18.0软件进行统计学分析。结果BGC823、SGC7901、AGS、MGC803、MKN45中的circ_0009910相对表达水平为(7.238±0.895)、(5.023±0.786)、(4.184±0.356)、(8.561±1.026)、(3.478±0.301),较正常胃癌显著升高(P<0.01),siRNA转染敲降circ_0009910后,BGC823、AGS细胞活力、集落形成数目、迁移和侵袭能力与对照组相比均明显降低(P<0.01),N-cadherin、Snail蛋白的相对表达降低,而E-cadherin表达增加。结论circ_0009910在胃癌细胞中高表达,可促进胃癌细胞增殖、集落形成、迁移和侵袭及EMT。

Functional mechanism of circ_0009910 in gastric cancer

Objective To explored the mechanism of circ_0009910 in gastric cancer cells by detecting and analyzing the expression of it.Methods qRT-PCR assay was used to detect the expression level of circ_0009910 in gastric cancer cell lines BGC823,SGC7901,AGS,MGC803 and MKN45.In circumstance,circ_0009910 siRNA was used to knock down circ_0009910 in BGC823 and AGS cells.The transfection efficiency was verified.In BGC823 and AGS cells knocked down by circ_0009910,cell proliferation was detected by MTT assay,colony formation was detected by plate assay,migration and invasion were detected by Transwell,and epithelial-mesenchymal transition(EMT)was detected by western-blotting.The software of SPPSS 18.0 was used to conduct statistical analysis.Results The relative expression levels of circ_0009910 in BGC823,SGC7901,AGS,MGC803,and MKN45 were(7.238±0.895),(5.023±0.786),(4.184±0.356),(8.561±1.026),(3.478±0.301),which were significantly higher than normal gastric cancer(P<0.01).After knocking down circ_0009910 by siRNA transfection,the BGC823,AGS cell viability,colony formation number,migration and invasion ability were significantly reduced compared with the control group(P<0.01),the relative expressions of N-cadherin and Snail proteins were reduced,and E-Cadherin expression increased.Conclusion Circ_0009910 is highly expressed in gastric cancer cells,and can promote gastric cancer cell proliferation,colony formation,migration,invasion and EMT.