Effects of tricalcium silicate cements on osteogenic differentiation of human bone marrow-derived mesenchymal stem cells in vitro |
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| Affiliation: | 1. Department of Dental and Biomedical Material Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan;2. Department of Oral Biology, Georgia Regents University, Augusta, GA, USA;3. Oral and Dental Research Division, Department of Surgery and Medicine, National Research Center, Egypt;4. Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi’an, China;5. Department of Stomatology, Fuzhou Dongfang Hospital, Fuzhou, China;6. Department of Graduate Studies, Georgia Regents University, Augusta, GA, USA;1. ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Mawson Institute, University of South Australia, South Australia, Australia;2. School of Mathematical Sciences, University of Adelaide, South Australia, Australia;1. Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi’an, China;2. Department of Oral Anatomy and Physiology and TMD, School of Stomatology, Fourth Military Medical University, Xi’an, China;3. Department of Prosthodontics, School of Stomatology, Peking University, Beijing, China;4. Department of Stomatology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, China;5. Department of Restorative Dentistry, Faculty of Dental Surgery, University of Malta, Malta;6. Department of Endodontics, College of Dental Medicine, Georgia Regents University, Augusta, GA, USA;7. College of Graduate Studies, Georgia Regents University, Augusta, GA, USA;1. Department of Orthopaedics Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, PR China;2. Department of Orthopedics, The First People’s Hospital of Zunyi, Zunyi 563000, PR China;3. Department of Oncology, Affiliated Hospital of Zunyi Medical College, Zunyi 563000, PR China;4. Department of Orthopedics, Guiyang Orthopedic Hospital, Guiyang 550000, PR China;5. Department of Emergency, Affiliated Hospital of Zunyi Medical College, Zunyi 563000, PR China;6. Spine Center, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, PR China;1. Centre for Translational Bone, Joint and Soft Tissue Research, Medical Faculty and University Hospital, Technische Universität Dresden, Dresden, Germany;2. Department of Trauma, Hand and Reconstructive Surgery, University Hospital Giessen-Marburg GmbH, Campus Giessen, Germany;3. Experimental Trauma Surgery, Justus-Liebig-University of Giessen, Germany;4. Leibniz Institute for Solid State and Materials Research (IFW), Dresden, Germany;5. INNOTERE GmbH, Radebeul, Germany;1. State Key Laboratory of Military Stomatology, School of Stomatology, The Fourth Military Medical University, Xi’an, China;2. Department of Stomatology, Affiliated Hospital of Academy of Military Medical Science, Beijing, China;3. Xijing Hospital, The Fourth Military Medical University, Xi’an, China;4. College of Dental Medicine, Georgia Reagents University, Augusta, GA, USA;1. Endodontic Clinical Section, Dental School, Department of Biomedical and NeuroMotor Sciences, University of Bologna, Bologna, Italy;2. Laboratory of Biomaterials and Oral Pathology, Dental School, Department of Biomedical and NeuroMotor Sciences, University of Bologna, Bologna, Italy |
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| Abstract: | Tricalcium silicate cements have been successfully employed in the biomedical field as bioactive bone and dentin substitutes, with widely acclaimed osteoactive properties. This research analyzed the effects of different tricalcium silicate cement formulations on the temporal osteoactivity profile of human bone marrow-derived mesenchymal stem cells (hMW-MSCs). These cells were exposed to four commercially available tricalcium silicate cement formulations in osteogenic differentiation medium. After 1, 3, 7 and 10 days, quantitative real-time polymerase chain reaction and Western blotting were performed to detect expression of the target osteogenic markers ALP, RUNX2, OSX, OPN, MSX2 and OCN. After 3, 7, 14 and 21 days, alkaline phosphatase assay was performed to detect changes in intracellular enzyme level. An Alizarin Red S assay was performed after 28 days to detect extracellular matrix mineralization. In the presence of tricalcium silicate cements, target osteogenic markers were downregulated at the mRNA and protein levels at all time points. Intracellular alkaline phosphatase enzyme levels and extracellular mineralization of the experimental groups were not significantly different from the untreated control. Quantitative polymerase chain reaction results showed increases in downregulation of RUNX2, OSX, MSX2 and OCN with increasing time of exposure to the tricalcium silicate cements, while ALP showed peak downregulation at day 7. For Western blotting, OSX, OPN, MSX2 and OCN showed increased downregulation with increased exposure time to the tested cements. Alkaline phosphatase enzyme levels generally declined after day 7. Based on these results, it is concluded that tricalcium silicate cements do not induce osteogenic differentiation of hBM-MSCs in vitro. |
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| Keywords: | Bone marrow Human origin Mesenchymal stem cells Osteogenic differentiation Tricalcium silicate cements |
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