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Spontaneous healing of the tympanic membrane after traumatic perforation in rats |
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| Affiliation: | 1. Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo (USP), Ribeirão Preto, SP, Brazil;2. Department of Cell and Molecular Biology and Pathogenic Bioagents, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo (USP), Ribeirão Preto, SP, Brazil |
| Abstract: | The most common etiologies of tympanic membrane perforation are infections and trauma.ObjectiveThe objective of the present study was to assess the healing of traumatic tympanic membrane perforation in rats.MethodsThe tympanic membrane from male Wistar rats was perforated in the anterior and posterior portions to the handle of the malleus. Five tympanic membranes were evaluated 3 days after tympanic perforation; 5 after 5 days; 5 after 7 days; 3 after 10 days; and 4 after 14 days. The tympanic membranes were submitted to histopathological evaluation after hematoxylin–eosin staining.ResultsTympanic membrane closure occurred at about 7–10 days after injury and the healing process was complete by day 14. The proliferative activity of the outer epithelial layer was present close to the handle of the malleus and to the tympanic annulus.ConclusionThe spontaneous healing process of the tympanic membrane starts from the outer epithelial layer, with later healing of the lamina propria and the mucosal layer. |
| Keywords: | Tympanic membrane perforation Wound healing Histology Perfuração da membrana timpânica Cicatrização Histologia |
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