| ISSR法鉴定中药黄精与卷叶黄精 |
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| 引用本文： | 周晔,王润玲,唐铖,安适之,姚智.ISSR法鉴定中药黄精与卷叶黄精[J].天津医科大学学报,2006,12(2):178-180,189. |
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| 作者姓名： | 周晔 王润玲 唐铖 安适之 姚智 |
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| 作者单位： | 1. 天津医科大学药学院,天津,300070
2. 天津医科大学免疫学教研室,天津,300070 |
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| 基金项目： | 天津市卫生局基金资助项目(03009)，天津医科大学科学基金资助项目(2003KY4) |
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| 摘 要： | 目的:鉴别中药黄精和卷叶黄精,探索中药鉴别的新方法。方法:从原植物鉴定、显微鉴定和ISSR分析等方面着手。对随机引物进行筛选,选出两个引物,对样品进行PCR扩增,用电泳分离产物。结果:通过引物(AC)8T,(AG)8T等经PCR扩增产生足够的多态性谱带用于分析。结论:ISSR技术可应用于黄精和卷叶黄精的区分。
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| 关 键 词： | 黄精 卷叶黄精 显微鉴定 简单重复序列间扩增多态性 |
| 文章编号： | 1006-8147（2006）02-0178-04 |
| 收稿时间： | 2006-03-29 |
| 修稿时间： | 2006-03-29 |
Identification of Polygonatum sibiricum and P. cirrhifolium by ISSR |
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| ZHOU Ye,WANG Run-ling,TANG Cheng,AN Shi-zhi,YAO Zhi.Identification of Polygonatum sibiricum and P. cirrhifolium by ISSR[J].Journal of Tianjin Medical University,2006,12(2):178-180,189. |
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| Authors: | ZHOU Ye WANG Run-ling TANG Cheng AN Shi-zhi YAO Zhi |
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| Institution: | 1. College of Pharmacy; 2. Department of Immunology, Tianjin Medical University, Tianjin 300070, China |
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| Abstract: | Objective: To identify Polygonatum sibiricum and its fake called P.cirrhifolium,and set up a new method.Methods: Character identification,microscopic identification and ISSR were used.Some arbitrary primers were evaluated,and two of them were selected for the research.All of the sample were amplified by PCR system and separated by electrophoresis.Results: The primers such as(AC)8T,(AG)8T could yield enough polymorphic bands for the analysis.Conclusion: The ISSR method we set up could be used for the identification of P.sibiricum and P.cirrhifolium. |
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| Keywords: | Polygonatum sibiricum Polygonatum cirrhifolium Microscopic identification ISSR |
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