骨疏灵的大鼠血清药物化学分析

目的:对骨疏灵血清药物化学进行研究,通过分析其入血成分探讨该复方发挥药效的物质基础,为骨疏灵的临床应用提供参考。方法:采用血清药物化学研究方法,建立骨疏灵体外和血清的全方、缺味组方及单味药材的HPLC,通过对比分析骨疏灵体外和血清的全方、缺味组方及单味药材的HPLC色谱峰,初步确定骨疏灵的入血成分、移行成分及各成分的主要归属。结果:骨疏灵灌胃后在大鼠体内发现21个入血成分,其中11个来自该复方的原型,4个是新产生的物质,其余均为代谢物。结论:骨疏灵入血成分是各味药材复方配伍后共同作用的结果,其中淫羊藿贡献最大,复方血清药物化学研究为深入阐述骨疏灵药效物质基础提供实验依据。

Serum Pharmacochemistry Analysis of Gushuling in Rats

Objective: To make a preliminary study on serum pharmacochemistry of Gushuling and investigate medicinal mechanism by a blood component analysis. Method: In vitro,in vivo HPLC of Gushuling,Gushuling without a certain receipt and every single receipt were established according to pharmacochemistry research method. And by comparing those HPLC,constituents absorbed into blood and components metabolized in blood were determined. Result: A total of 21 components in serum were determined after intragastric administration of Gushuling in rats,11 of them were prototype compound of preparation,4 of them were constituents generated by receipts interaction in vivo and the rest were metabolites. Conclusion: Components of Gushuling in rat serum are generated by interaction of all receipts,and the greatest contribution come from Epimedii Folium.This pharamcochemisty study of Gushuling provides a substance foundation for further pharmacodynamic mechanism of compound.