新痹痛灵抗炎作用及其机制

目的:研究新痹痛灵(New Bitongling,NBTL)的抗炎作用,揭示其抗炎作用及机制。方法:体内实验取30只SD雄性大鼠,随机分成模型组、阳性药布洛芬缓释胶囊组(0.054 g·kg-1)以及新痹痛灵高剂量组(11 g·kg-1,简称NBTLH)、低剂量组(2.75 g·kg-1,简称NBTLL);连续ig给药5 d,1次/d;末次给药1 h后,复制组胺致大鼠毛细血管通透性增加模型、采用尾静脉注射伊文斯蓝后提取皮肤中染料、测定提取液590 nm的吸光度反映毛细血管通透性;复制角叉菜胶致大鼠足跖肿胀模型、采用容积测量法观察各组大鼠足跖肿胀度;体外实验以脂多糖(LPS)诱导的RAW 264.7细胞分泌炎性因子复制炎症模型,酶联免疫吸附试验(ELISA)法测定各组细胞上清液中前列腺素E2(PGE2)含量。结果:与模型组比较,布洛芬缓释胶囊组,NBTLH,NBTLL组伊文斯蓝含量明显降低(P0.01);表明均能抑制大鼠毛细血管通透性增加;与模型组相比,在相同时间点,NBTLH组大鼠足跖肿胀度均降低(各时间点P0.05),NBTLL在各个时间点都有抑制趋势,但无显著性差异;与正常组比较,模型组的PGE2表达水平升高(P0.05);与模型组比较,NBTLH,NBTLL组PGE2表达水平显著降低(P0.05,P0.01)。结论:NBTL对炎症有明显抑制作用,其抗炎作用可能与抑制炎症组织PGE2的合成或分泌有关。

Anti-inflammatory Effect of New Bitongling Compound and Its Mechanism

Objective: To investigate the anti-inflammatory effect and mechanism of New Bitongling compound(NBTL). Method: The 30 SD male rats were randomly divided into four groups:the model group, Bloven sustained release capsules positive control group(0.054 g·kg^-1), New Bitongling high-dose group and low-dose group(NBTLH, NBTLL, 11,2.75 g·kg^-1). Rats were gave ig administered for 5 days, once a day. After 1 h of the last administration,models of increased capillary permeability (induced by histamine) were established, then dye in the skin was extracted after intravenous injection with Evans blue (EB)in tails, and the absorbance of the skins supernatant fluid at 590 nm was determined to react the permeability of skin capillaries indirectly.The pedal swelling model induced by carrageenan were established,and the swelling degree of the paw in various groups was observed using method of volume measurement. Inflammation model in vitro was established using RAW264.7 cells secrete inflammatory factors induced by lipopolysaccharide(LPS), and prostaglandin E₂(PGE₂) content in cell supernatant of various groups was determined using enzyme-linked immunosorbant assay(ELISA) method. Result: Compared with model group, the level of EB was decreased in Bloven sustained release capsules group, NBTLH and NBTLL groups(P<0.01), indicating that they can inhibit the increase of blood capillary permeability in rats. Compared with model group, the swelling of foot plantar in NBTLH group rats was relieved at the same time points (each time point, P<0.05).While the NBTLL group showed tendency of inhibition at various time points, but with out statistical significance.Compared with normal group,the expressions of PGE₂ were elevated in the model group(P<0.05).Compared with model group,the expressions of PGE₂ were significantly reduced in NBTLH and NBTLL groups(P<0.05, P<0.01). Conclusion: NBTL has an anti-inflammatory effect, which may be associated with suppressing synthesis or secretion of PGE₂ in inflammatory tissues.