星点设计-效应面法优化牛蒡子中牛蒡子苷和牛蒡子苷元提取工艺

目的：优化牛蒡子中双指标成分最佳提取工艺。方法：采用星点设计-效应面法，在3个主要因素作用下，以牛蒡子苷及牛蒡子苷元提取率为评估指标，经过多元线性回归和二项式拟合，利用岭嵴分析法优化牛蒡子苷及其苷元提取工艺。结果：确定牛蒡子苷及牛蒡子苷元最佳提取工艺为：70%乙醇，24倍量，超声提取15 min。结论：上述工艺能够大量提取牛蒡子苷及牛蒡子苷元，为制备牛蒡子苷及其苷元提供试验依据，也为充分开发利用牛蒡资源提供参考。

Optimization of Extraction Process for Arctiin and Arctigenin in Arctium lappa L. Based on Central Composite Design and Response Surface Methodology

This study was aimed to optimize the extraction process of double-marker components for Arctium lappa L. The central composite design and response surface methodology was used. According to 3 main factors, the extraction rates of arctiin and arctigenin was used as evaluation indexes. Multiple linear regression and two-order polynomial equation were used. The binomial fitting model was performed in the optimization of arctiin and arctigenin extraction technology. The results showed that the indentified optimized extraction technology of arctiin and arctigenin was 70% ethanol, 24-fold, ultrasonic solvent extraction for 15 minutes. It was concluded that this technology was able to extract large amount of arctiin and arctigenin, which provided experiment evidences for arctiin and arctigenin preparation. It also provided references for the development and utilization of arctiin and arctigenin.