| Cyclopiazonic acid增加SHR和WKY血管平滑肌细胞Ca~(2+)-依赖性外向K~+-电流 |
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| 引用本文： | 关永源,John Zhang,Lee RMKW,Kwan CY,Daniel EE. Cyclopiazonic acid增加SHR和WKY血管平滑肌细胞Ca~(2+)-依赖性外向K~+-电流[J]. 中国药理学通报, 1993, 0(4) |
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| 作者姓名： | 关永源 John Zhang Lee RMKW Kwan CY Daniel EE |
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| 作者单位： | 中山医科大学药理教研室，Section of Neurosurgery，Medical Center，The University of Chicago，Chicago，Illinois 60637 USA，Smooth Musele Research Program，Departments of Anacthesia and Biomedical Sciences，McMaster University，Hamilton，Ontario Canada L8N 3Z5，Smooth Musele Research Program，Departments of Anacthesia and Biomedical Sciences，McMaster University，Hamilton，Ontario Canada L8N 3Z5，Smooth Musele Research Program，Departments of Anacthesia and Biomedical Sciences，McMaster University，Hamilton，Ontario Canada L8N 3Z5 广州510089 |
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| 基金项目： | 中国国家自然科学基金 No 39070931，加拿大 Heart and Stroke Foundation of Ontario提供资助 |
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| 摘 要： | 采用全细胞及细胞贴附式斑片钳技术记录自发性高血压大鼠(SHR)和Wistar-Kyoto对照鼠(WKY)培养主动脉平滑肌细胞的Ca~(2+)-依赖性外向K~+电流[I_(k(Ca))],测定肌浆网Ca~(2+)泵抑制剂CPA对其影响.CPA能增加I_K(Ca))单通道开放时间,缩短关闭时间,增加全细胞I_(K(Ca))幅度,这些作用与Ca~(2+)相关并可被K~+通道阻断药glybenclamide阻断。CPA作用在SHR和WKY之间无明显差异。结果提示高血压状态下血管平滑肌的功能改变可能与I_(K(Ca))无关。
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| 关 键 词： | cyclopiazonic acid K~+-通道 血管平滑肌 |
Cyclopiazonic acid enhanced the Ca~(2+)-dependent outward K~+ currents in cultural aortic smooth muscle cells from SHR and WKY |
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| GUAN Yong-Yuan,John Zhang,Lee RKW,Kwan CY,Daniel EE. Cyclopiazonic acid enhanced the Ca~(2+)-dependent outward K~+ currents in cultural aortic smooth muscle cells from SHR and WKY[J]. Chinese Pharmacological Bulletin, 1993, 0(4) |
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| Authors: | GUAN Yong-Yuan John Zhang Lee RKW Kwan CY Daniel EE |
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| Affiliation: | GUAN Yong-Yuan,John Zhang1,Lee RKW2,Kwan CY2,Daniel EE2 |
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| Abstract: | ABSTRACT The effects of cyclopiazonic acid (CPA) on the Ca2+ -dependent ourward K+-cur-rent [IK(ca)] were studied using whole-cell and single-channel patch-clamp techniques. Depolarization (pipette potential range from - 20 to - 120 mV)induced a outward Ik(ca) with a conductance of about 40 pS in the cultural aortic smooth muscle cells from SHR and WKY. 10 μmol · L-1 CPA significantly enhanced these currents and prolonged the mean open time of the channels. This effect of CPA was completely blocked by glybenclamide, a K+-channel block-er. In the whole cell recording experiments, CPA increased the amplitude of outward K+-current. This effect of CPA was Ca2+-dependent and completely blocked by glybenclamide. There was no any significant difference between the effects of CPA in SHR and in WKY. These results suggest that the functional change of vascular smooth muscle in SHR doesn't appear to be related to Ik(ca). |
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| Keywords: | potassium channels cyclopiazonic acid vascular smooth muscle |
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