| 枳椇子中总黄酮的提取工艺优化 |
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| 引用本文： | 文为,张洪,曾嵘. 枳椇子中总黄酮的提取工艺优化[J]. 中国药师, 2007, 10(10): 965-967 |
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| 作者姓名： | 文为 张洪 曾嵘 |
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| 作者单位： | 武汉大学人民医院,武汉,430060 |
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| 摘 要： | 目的:优选枳楔子提取物的最佳提取工艺。方法:以提取物得率及提取物中总黄酮含量为检测指标,对提取溶剂进行筛选;通过考察小鼠肝匀浆中的SOD活性、MDA含量,确定最佳提取方法。同时采取正交设计法对最佳提取方法的提取工艺进行了考察。结果:以75%乙醇为提取溶剂,采用热回流提取法,乙醇浓度为75%、提取时间2h、乙醇加入量为8倍量时为最佳提取工艺。结论:该优选提取工艺合理、可行、可控。
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| 关 键 词： | 枳椇子 提取工艺 正交设计法 总黄酮 |
| 文章编号： | 1008-049X（2007）10-0965-03 |
| 修稿时间： | 2007-03-12 |
Optimization the Extracting Conditions of Total Flavonoids in Hovenia Dulcis Thunb Zhijuzi by Alcohol |
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| Wen Wei,Zhang Hong,Zeng Rong. Optimization the Extracting Conditions of Total Flavonoids in Hovenia Dulcis Thunb Zhijuzi by Alcohol[J]. China Pharmacist, 2007, 10(10): 965-967 |
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| Authors: | Wen Wei Zhang Hong Zeng Rong |
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| Affiliation: | Renmin Hospital of Wuhan University, Wuhan 430060, China |
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| Abstract: | Objective:To optimize the extracting condition of total flavonoids in Hovenia dulcis Thunb by alcohol.Method:The extractive yield and total flavonoids were used to screen the extracting dissolvent.The SOD activity and the concentration of MDA in rats liver homogenate were investigated to ascertain the extracting conditions.The extracting process was determined by orthogonal de- sign.Result:The optimal extracting condition was heat reflux for 2 hours,8-fold 75% alcohol was used.Conclusion:The process se- lected in this experiment is reasonable,practical and controllable. |
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| Keywords: | Hovenia dulcis Thunb Extracting technology Orthogonal design methods Total flavonoids |
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