| 25羟维生素D3对支气管上皮细胞维生素D受体表达及分布的影响 |
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| 引用本文： | Dong H,Zhao H,Liu L,Liang Z,Lv Y,Cai S. 25羟维生素D3对支气管上皮细胞维生素D受体表达及分布的影响[J]. 南方医科大学学报, 2012, 32(1): 28-31 |
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| 作者姓名： | Dong H Zhao H Liu L Liang Z Lv Y Cai S |
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| 作者单位： | 南方医科大学南方医院呼吸科 |
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| 基金项目： | 国家自然科学基金(30971328);教育部博士点基金(20094433110011);南方医院院长基金(2010Z002)~~ |
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| 摘 要： | 目的探讨25羟维生素D3对人正常气道上皮维生素D受体表达及分布的影响。方法选取正常人支气管上皮细胞系16HBE为研究对象,MTT法分别检测不同浓度25羟维生素D3对细胞活力的影响;实验组分为对照组和不同浓度25羟维生素D3处理组。各处理因素作用细胞时间为24 h。用实时荧光定量PCR、Western blot、免疫荧光法观测各处理组维生素D受体(VDR)mRNA及蛋白表达、分布的情况。结果与对照组相比,各个不同处理浓度的25羟维生素D3对VDR的表达量及分布均无显著增加(P>0.05)。结论 25羟维生素D3对支气管上皮细胞的影响可能并不依赖于VDR的表达及转位。
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| 关 键 词： | 人气道上皮细胞 25羟维生素D3 维生素D受体 |
Effect of 25-hydroxyvitamin D(3) on vitamin D receptor expression and distribution in human bronchial epithelial cells in vitro |
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| Dong Hangming,Zhao Haijin,Liu Laiyu,Liang Zhenyu,Lv Yanhua,Cai Shaoxi. Effect of 25-hydroxyvitamin D(3) on vitamin D receptor expression and distribution in human bronchial epithelial cells in vitro[J]. Journal of Southern Medical University, 2012, 32(1): 28-31 |
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| Authors: | Dong Hangming Zhao Haijin Liu Laiyu Liang Zhenyu Lv Yanhua Cai Shaoxi |
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| Affiliation: | Department of Respiratory Diseases, Southern Medical University, Guangzhou, China. huxidhm@126.com |
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| Abstract: | Objective To evaluate the effect of 25-hydroxyvitamin D3 on the expression and distribution of vitamin D receptor in normal human bronchial epithelial cells.Methods MTT assay was used to assess the viability of human airway epithelial cell line 16HBE following a 24-h exposure to different concentrations of 25-hydroxyvitamin D3.Real-time quantitative PCR,Western blotting,and immunofluorescence assay were used to observe the expression and distribution of vitamin D receptor in the cells following the exposure.Results Compared with the control cells,16HBE cells exposed to different concentrations of 25-hydroxyvitamin D3 exhibited no significantly increase in the expression or distribution of vitamin D receptor.Conclusion The influence of 25-hydroxyvitamin D3 on bronchial epithelial cells might be independent of the expression and translocation of vitamin D receptor. |
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| Keywords: | human airway epithelium 25-hydroxy vitamin D3 vitamin D receptor |
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