南瓜提取物对小鼠铅诱导脂质过氧化及抗氧化酶活力的影响

背景铅是一种广泛存在的重金属毒物,可影响机体的抗氧化酶系统,降低超氧化物歧化酶(superoxide dismutase,SOD)及谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)的活性,造成肝、肾、脑等损害.南瓜在防治糖尿病、降脂、防癌、清除重金属毒物等方面具有广泛的应用价值.目的探讨染铅小鼠抗氧化系统的改变及南瓜提取物(pumpkin distillable subject,PKD)对抗氧化酶系统的影响.设计随机对照的实验研究.地点、材料和干预本实验在哈尔滨医科大学公共卫生学院动物室完成.选用纯种雄性健康昆明鼠60只,购自中国药品生物制品检定所实验动物中心.分为正常对照组、铅处理组和联合处理组,对照组小鼠饮正常水,联合处理组小鼠经饮水铅染毒,造成铅中毒模型后给予南瓜提取物,铅处理组不给南瓜提取物,4周后处死,取血清和肝脏并制备匀浆.测定血清和肝匀浆中SOD,GSH-Px活力及丙二醛含量.主要观察指标各组小鼠血清和肝匀浆中SOD,GSH-Px活力及丙二醛含量.结果联合处理组血清和肝匀浆中SOD活性明显高于铅处理组(q=7.83,6.01,P＜0.01),丙二醛含量明显低于铅处理组(q=6.75,6.51,P＜0.01).铅处理组小鼠血清和肝匀浆中GSH-Px含量(3 746.42±669.30)μkat/L,(702.64±139.36)nkat/g]比正常对照组(6242.92±940.02)μkat/L,(1 098.05±196.37)nkat/g]明显降低(q=7.90,5.59,P＜0.01).结论铅中毒可引起小鼠过氧化损伤,南瓜提取物可以减轻铅中毒引起的脂质过氧化,提高小鼠机体的抗氧化能力.

Effect of pumpkin distillable subject on lipid peroxidation and the activity of antioxidative enzyme induced by Plumbum in mouse

BACKGROUND:Plumbum(Pb) is a heavy metal widely existed, which would affect the antioxidative enzymic system of the body, and to decrease the activities of superoxide dismutase(SOD) and glutathione peroxidase (GSH-Px), and thereby to damage liver, kidney and brain, etc.Pumpkin has wide applicable merit in preventing diabetes, against hyperlipemia, preventing cancer and eliminating heavy metal poising, etc.OBJECTIVE:To investigate the changes of antioxidative enzymic system induced by Pb in mouse as well as the effect of Pumpkin Distillable Subject (PKD) on antioxidation enzymic system.DESIGN:A randomized controlled study.SETTING, PARTICIPANTS and INTERVENTIONS:Our study was conducted in animal laboratory of the Faculty of Public Health of Harbin Medical University.60 purebred male healthy Kunming mice were obtained from the Experimental Animal Center of China Pharmaceutical Biological Product Analysis Institute, which were allocated into normal control group (control group), Pb management group(Pb group) and combined management group (PKD group) .Mice in control group drank normal water,mice in Pumpkin group drank water that polluted by Pb to establish Plumbism model and then provided with PKD, and mice in Pb group only drank Pb-polluted water without PKD management.All animals were executed after 4 weeks for drawing bloods and taking liver, which would be prepared into homogenate.The activities of SOD, GSH-Px as well as the content of MDA in serum and hepatic homogenate were assayed.MAIN OUTCOME MEASURES:The activities of SOD, GSH-Px as well as the content of MDA in serum and hepatic homogenate in mice of each group were assayed.RESULTS:The serous and hepatic activities of SOD, GSH-Px in mice of PKD group were significantly higher than that of Pb group( q =7.83, 6.01,P ＜ 0.01 ), but the concentrations of MDA were significantly lower that that of Pb group( q = 6.75, 6.51, P ＜ 0.01).The serous and hepatic activities of SOD and GSH-Px in mice of Pb group (3 746.42 ±669.30) μ kat/L,(702.64 ± 139.36) nkat/g]were significantly lower than that (6 242.92 ±940.02) μkat/L, (1 098.05±196.37) nkat/g] of control group(q=7.90,5.59, P ＜0.01).CONCLUSION:Plumbism could induce peroxidative injury in mouse.PKD could reduce the lipid peroxidation induced by Pb and increase antioxidative ability in mice.