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| 放疗联合周剂量重组人血管内皮抑素对A549肺腺癌裸鼠移植瘤生长抑制作用的研究 | |
| 引用本文： | 蒋晓东,戴鹏,吴瑾,宋大安,于金明. 放疗联合周剂量重组人血管内皮抑素对A549肺腺癌裸鼠移植瘤生长抑制作用的研究[J]. 肿瘤, 2010, 30(11). DOI: 10.3781/j.issn.1000-7431.2010.11.005 |
| 作者姓名： | 蒋晓东 戴鹏 吴瑾 宋大安 于金明 |
| 作者单位： | 1. 天津医科大学研究生院,天津,300021;连云港市第一人民医院肿瘤科,连云港,222002 2. 连云港市第一人民医院肿瘤科,连云港,222002 3. 山东省肿瘤医院放射治疗科,济南,250117 |
| 基金项目： | 1.江苏省自然科学基金面上项目(编号:bk2009167)2.连云港市科技局资助课题项目 |
| 摘 要： | 目的:研究放疗联合周剂量重组人血管内皮抑素(recombinant human endostatin, rh-Endostatin)对肺腺癌A549裸鼠移植瘤生长的抑制作用.方法: 40 只裸鼠A549细胞移植瘤模型随机分成4组:空白对照组、rh-Endostatin治疗组、放射治疗组和放疗联合rh-Endostatin治疗组,观察并绘制肿瘤生长曲线图,计算肿瘤体积抑制率;肿瘤组织行常规HE病理学检查,免疫组织化学法检测肿瘤组织中微血管内皮CD31的表达及肿瘤微血管密度(microvessel density, MVD)的变化;采用免疫组织化学及Western印迹法检测血管内皮生长因子(vascular endothelial growth factor, VEGF)的表达;TUNEL法检测肿瘤细胞的凋亡. 结果:治疗第8天起,放疗联合rh-Endostatin治疗组的肿瘤体积与对照组相比,差异有统计学意义(P＜0.05).15 d后,rh-Endostatin治疗组、放射治疗组和放疗联合rh-Endostatin治疗组小鼠的肿瘤体积的抑制率依次为68.35%、90.78%和106.56%; rh-Endostatin治疗组小鼠的MVD较放射治疗组下降明显(P＜0.05);但rh-Endostatin治疗组与对照组、放疗联合rh-Endostati治疗组及放射治疗组相比,VEGF的改变差异均无统计学意义;放疗联合rh-Endostatin治疗组细胞凋亡明显.结论:放疗联合周剂量rh-Endostatin能抑制肿瘤的生长,较早诱导肿瘤退缩,可能与减少放射治疗后肿瘤血管再生及增加肿瘤细胞和内皮细胞的凋亡相关;各治疗组小鼠均未出现急性不良反应,因此该方法具有短疗程的优势,可用于临床推广. |
| 关 键 词： | 癌,非小细胞肺 血管抑制蛋白质类 动物实验 小鼠,裸 A549细胞 |
Inhibitory effects of radiotherapy combined with weekly application of recombinant human endostatin on the human pulmonary adenocarcinoma A549 xenografts in nude mice |
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| JIANG Xiao-dong,DAI Peng,WU Jin,SONG Da-an,YU Jin-ming. Inhibitory effects of radiotherapy combined with weekly application of recombinant human endostatin on the human pulmonary adenocarcinoma A549 xenografts in nude mice[J]. Tumor, 2010, 30(11). DOI: 10.3781/j.issn.1000-7431.2010.11.005 | |
| Authors: | JIANG Xiao-dong DAI Peng WU Jin SONG Da-an YU Jin-ming |
| Affiliation: | JIANG Xiao-dong 1,2,DAI Peng 2,WU Jin 2,SONG Da-an 2,YU Jin-ming 3 (1.Graduate School,Tianjin Medical University,Tianjin 300021,China,2.Department of Oncology,Lianyungang First People's Hospital,Lianyungang 222002,3.Department of Radiation Oncology,Shandong Cancer Hospital,Jinan 250117,China) |
| Abstract: | Objective:To investigate the inhibitory effects of radiotherapy combined with weekly application of recombinant human endostatin(rh-Endostatin) on the growth of human pulmonary adenocarcinoma A549 xenografts in nude mice.Methods:The xenografted model of A549 cells was established in nude mice.The 40 nude mice were randomly divided into 4 groups: control group,single rh-Endostatin group,single radiotherapy group,and radiotherapy plus rh-Endostatin.These nude mice were observed for 15 d.Tumor growth curve was... |
| Keywords: | Carcinoma non-small cell lung Angiostatic proteins Animal experimentation Mice nude A549 cells |
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