| 垂盆草醇提物抑制HepG2细胞STAT-3信号通路诱导细胞凋亡的研究 |
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| 引用本文： | 曾军英,李胜华,伍贤进,刘丹,万雄.垂盆草醇提物抑制HepG2细胞STAT-3信号通路诱导细胞凋亡的研究[J].中国中药杂志,2014,39(17):3349-3352. |
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| 作者姓名： | 曾军英 李胜华 伍贤进 刘丹 万雄 |
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| 作者单位： | 民族药用植物资源研究与利用湖南省重点实验室 怀化学院 生命科学系, 湖南 怀化 418008;民族药用植物资源研究与利用湖南省重点实验室 怀化学院 生命科学系, 湖南 怀化 418008;民族药用植物资源研究与利用湖南省重点实验室 怀化学院 生命科学系, 湖南 怀化 418008;民族药用植物资源研究与利用湖南省重点实验室 怀化学院 生命科学系, 湖南 怀化 418008;民族药用植物资源研究与利用湖南省重点实验室 怀化学院 生命科学系, 湖南 怀化 418008 |
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| 基金项目： | 湖南省教育厅平台项目（11K052，09K105）；湖南省高等学校产业化培育项目（13cy026）；湖南省科技计划项目（2013FJ6090）；民族药用植物资源研究与利用湖南省重点实验室开放基金项目（HHUW2010-65）；湖南省植物学“十二五”重点建设学科（湘教发[2011]42号） |
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| 摘 要： | 目的:研究垂盆草醇提物对STAT-3信号通路的影响,及其诱导细胞凋亡的分子机制。方法:MTT法检测垂盆草醇提物对HepG2细胞增殖的影响;流式细胞术分析结合FITC-Annexin V/PI双标记检测对肝癌细胞凋亡的影响,免疫印迹试验检测细胞凋亡Caspase-3,Caspase-9,PARP,P-STAT-3(Tyr705),STAT-3,Bcl-2,Mcl-1相关蛋白表达水平。结果:垂盆草醇提物可明显抑制HepG2细胞的增殖,诱导HepG2细胞凋亡,且抑制作用呈剂量依赖效应。垂盆草醇提物作用后,抑制STAT-3信号转导通路,下调Mcl-1和Bcl-2的表达,引起凋亡相关蛋白Caspase-3,Caspase-9的降解/活化及PARP的降解,并呈剂量依赖效应。结论:垂盆草醇提物通过抑制STAT-3信号转导通路和Mcl-1和Bcl-2的表达,进而抑制HepG2细胞的增殖,诱导其凋亡。
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| 关 键 词： | 垂盆草 细胞增殖 细胞凋亡 STAT-3 Mcl-1 Bcl-2 信号转导 HepG2细胞株 |
| 收稿时间： | 2013/12/4 0:00:00 |
Study on impact of ethanol extracts from Sedum sarmentosum in inhibiting STAT-3 signaling and inducing apoptosis of human hepatocellular carcinoma cell line HepG2 |
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| ZENG Jun-ying,LI Sheng-hu,WU Xian-jin,LIU Dan and WAN Xiong.Study on impact of ethanol extracts from Sedum sarmentosum in inhibiting STAT-3 signaling and inducing apoptosis of human hepatocellular carcinoma cell line HepG2[J].China Journal of Chinese Materia Medica,2014,39(17):3349-3352. |
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| Authors: | ZENG Jun-ying LI Sheng-hu WU Xian-jin LIU Dan and WAN Xiong |
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| Institution: | Key Laboratory of Hunan Province for Study and Utilization of Ethnic Medicinal Plant Resources, Department of Life Sciences, Huaihua University, Huaihua 418008, China;Key Laboratory of Hunan Province for Study and Utilization of Ethnic Medicinal Plant Resources, Department of Life Sciences, Huaihua University, Huaihua 418008, China;Key Laboratory of Hunan Province for Study and Utilization of Ethnic Medicinal Plant Resources, Department of Life Sciences, Huaihua University, Huaihua 418008, China;Key Laboratory of Hunan Province for Study and Utilization of Ethnic Medicinal Plant Resources, Department of Life Sciences, Huaihua University, Huaihua 418008, China;Key Laboratory of Hunan Province for Study and Utilization of Ethnic Medicinal Plant Resources, Department of Life Sciences, Huaihua University, Huaihua 418008, China |
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| Abstract: | Objective: To investigate the impact of ethanol extracts from Sedum sarmentosum (ESB) on STAT-3 signaling and its probable molecular mechanism in inducing apoptosis. Method: MTT assay was used to detect the impact of ESB on HepG2 cell proliferation. FITC-Annexin V-FITC/PI double-labeling were used to investigate the impact on hepatoma carcinoma cell apoptosis. Western blot analysis was used to test the expression levels of cell apoptosis-related proteins Caspase-3, Caspase-9, PARP, P-STAT-3(Tyr705), STAT-3, Bcl-2, Mcl-1. Result: ESB could notably inhibit proliferation of HepG2 cells, and induce HepG2 cell apoptosis, with the dose-dependent inhibitory effect. In addition, ESB could inhibit STAT-3 signaling, down-regulate Mcl-1 and Bcl-2 expressions, and induce degradation/activation of apoptosis-related proteins Caspase-3 and Caspase-9 and PARP degradation in a dose-dependent manner. Conclusion: ESB inhibits HepG2 cell proliferation and induces apoptosis by inhibiting STAT-3 signaling and Mcl-1 and Bcl-2 expressions. |
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| Keywords: | Sedum sarmentosum cell proliferation cell apoptosis STAT-3 Mcl-1 Bcl-2 signal transduction HepG2 cell line |
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