| 7种海洋矿物药的比较分析研究 |
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| 引用本文： | 司玮,阿如娜,李尚蓉,张静娴,吴婉莹,崔亚君. 7种海洋矿物药的比较分析研究[J]. 中国中药杂志, 2014, 39(17): 3321-3325 |
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| 作者姓名： | 司玮 阿如娜 李尚蓉 张静娴 吴婉莹 崔亚君 |
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| 作者单位： | 上海中医药大学 中药学院, 上海 201203;中国科学院 上海药物研究所, 上海中药现代化研究中心, 上海 201203;上海中医药大学 中药学院, 上海 201203;中国科学院 上海药物研究所, 上海中药现代化研究中心, 上海 201203;中国科学院 上海药物研究所, 上海中药现代化研究中心, 上海 201203;中国科学院 上海药物研究所, 上海中药现代化研究中心, 上海 201203;上海中医药大学 中药学院, 上海 201203 |
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| 基金项目： | 国家高技术研究发展计划（863）项目（2013AA093002）；2015 年版药典标准提高项目 |
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| 摘 要： | 《中国药典》2010年版收载的海洋矿物药主要包括珍珠、珍珠母、蛤壳、牡蛎、瓦楞子、海螵蛸、石决明等,该类药材化学成分组成相似,主要是碳酸钙和少量的蛋白质。该文采用EDTA络合滴定法测定碳酸钙的含量;采用X射线粉末衍射,测定其碳酸钙晶型;采用紫外分光光度法对水解后样品中总氨基酸进行测定。通过系统的比较研究,寻找该类药材的差异性,结果显示,所收集的样品中牡蛎所含碳酸钙为三方晶系方解石,石决明及珍珠母晶型为三方晶系方解石与斜方晶系文石混合晶型,其余4种均为斜方晶系文石;所有样品的碳酸钙质量分数在86%~96%;海螵蛸中总氨基酸质量分数最高达1.70%、蛤壳中最低,平均为0.16%。通过对7种海洋矿物药中碳酸钙和总氨基酸的分析,建立了一种对海洋矿物药质量控制的有效方法。
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| 关 键 词： | 海洋矿物药 x射线衍射 碳酸钙 氨基酸 EDTA络合滴定 紫外分光光度法 |
| 收稿时间： | 2014-04-26 |
Comparative analysis of seven marine biological source of mineral drugs |
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| SI Wei,A Ru-n,LI Shang-rong,ZHANG Jing-xian,WU Wan-ying and CUI Ya-jun. Comparative analysis of seven marine biological source of mineral drugs[J]. China Journal of Chinese Materia Medica, 2014, 39(17): 3321-3325 |
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| Authors: | SI Wei A Ru-n LI Shang-rong ZHANG Jing-xian WU Wan-ying CUI Ya-jun |
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| Affiliation: | Shanghai University of Traditional Chinese Medicine College of Traditional Chinese medicine, Shanghai 201203, China;National Engineering Laboratory for TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;Shanghai University of Traditional Chinese Medicine College of Traditional Chinese medicine, Shanghai 201203, China;National Engineering Laboratory for TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;National Engineering Laboratory for TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;National Engineering Laboratory for TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;Shanghai University of Traditional Chinese Medicine College of Traditional Chinese medicine, Shanghai 201203, China |
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| Abstract: | The marine biological source of mineral drugs recorded in Chinese Pharmacopoeia (2010 version) mainly including pearl, nacre, clam shell, common oyster shell, ark shell, cuttle bone, and sea-ear shell are widely used in clinical. Calcium carbonate and a small amount of protein are the main components in this type of drugs. In this paper, a systematical and comparable study were carried out by determination of calcium carbonate by EDTA titration method, the crystal of calcium carbonate by X-Ray powder diffraction and the total amino acids (TAAs) of the hydrolyzed samples by ultraviolet spectrophotometry method. As a result, the crystal structure is calcite for common oyster shell, mixture of calcite and aragonite for nacre and sea-ear shell, aragonite for the other drugs. The content of calcium carbonate ranged from 86% to 96%. Cuttle bone has the highest amount of TAAs among the seven drugs which reached 1.7% while clam shell has the lowest content of 0.16% on average. In conclusion, an effective method was developed for the quality control of marine mineral drugs by comprehensive analysis of calcium carbonate and TAAs in the seven marine mineral drugs. |
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| Keywords: | marine biological source of mineral drugs X-ray powder diffraction calcium carbonate total amino acids EDTA titration method ultraviolot spectrophotometry method |
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