补肾化瘀方药调整免疫衰老的机理研究

本实验从三方面研究八味丹坤调整免疫衰老与改善自由基代谢的关系。结果表明老年小鼠白细胞介素２（ＩＬ－２）产生降低，过氧化脂质（ＬＰＯ）含量增加，过氧化氢酶（ＣＡＴ）活性下降，给药后上述指标均得到调整，臭氧入小鼠的上述指标出现类似衰老的变化，给药后，ＬＰＯ含量降低，同时ＣＡＴ和ＩＬ－２活性得到增强，且两者呈线性相关，在脾淋巴细胞培养液中另入过氧化氢（Ｈ２Ｏ２），发现可使淋巴细胞ＩＬ－２产生明显减少，同

Mechnism of Tonifying Kidney and Removing Blood Stasis Recipe in Modulation of Immune Senescence

This experiment was from 3 aspects to study the relationship between modulating immune senescence and the improvement of free radical metabolism by the recipe of Baweidankun decoction(BWDKD).The results showed that the production of IL-2 decreased,the content of LPO increased and the activity of catalase (CAT) declined in old mice.After medication of BWDKD the above mentioned indices were modulated.The ozone inhaled mice revealed similar changes of aging in the above indices.After BWDKD administration,the content of LPO decreased,meanwhile, the activity of CAT and IL-2 were strengthened and showed linear correlation.It was discovered that the production of IL-2 decreased significantly after adding H2O2 into the culture of splenic lymphocytes,but when BWDKD was added simultaneously,the IL-2 production was restored to the level of control group,in which no H2O2 was added.The results suggested that the modulating effect of BWDKD on immune senescence was closely related with the improvement of free radical metabolism,and it provided a partial evidence for the viewpoint of "Vitality deficiency with blood stasis is the principal mechanism of senescence