RNA干扰VEGF-C基因下调COX-2、Bcl-2表达对乳腺癌细胞凋亡的影响

 目的 利用人血管内皮生长因子-C（vascular endothelial growth factor-C，VEGF-C）小RNA干扰（siRNA）表达载体——重组质粒pSilencer3.0-VEGF-C/siRNA，研究VEGF-C基因下调抑制COX-2、Bcl-2表达及对人乳腺癌细胞株MDA-MB-435的体外作用。方法 pSilencer3.0-VEGF-C/siRNA表达质粒和阴性对照质粒稳定转染人乳腺癌细胞株MDA-MB-435、RT-PCR和Western blot检测转染前后VEGF-C、COX-2基因和VEGF-C、COX-2、Bc1-2蛋白的表达；MTT法和流式细胞仪检测VEGF-C RNA干扰对细胞的作用。结果 pSilencer3.0-VEGF-C/siRNA转染乳腺癌细胞MDA-MB-435、VEGF-C基因和蛋白水平表达量明显降低，COX2和Bcl-2表达下调，细胞体外活性下降，72 h后凋亡率达60%。结论 针对人VEGF-C基因的siRNA表达载体抑制VEGF-C表达的同时下调COX-2和Bcl-2的表达，促进乳腺癌细胞MDA-MB-435的凋亡。

Vascular endothelial cell growth factor-C small RNA interfering vector down-regulate COX-2,Bcl-2 and induce apoptosis in human breast cancer cells

Objective To clone the recombinant plasmid silencing vascular endothelial growth factor-C (VEGF-C) gene by small interfering RNA (siRNA) and observe its role in the expression of COX-2,Bcl-2 and proliferation in human breast cancer cells MDA-MB-435. Methods Breast cancer cells MDA-MBA-435 were transfected stably with the recombinant siRNA plasmid and negative control by positive liposomal methods.We examined the gene expression of VEGF-C,COX-2 by RT-PCR and protein expression of VEGF-C,COX-2 and Bcl-2 by We...