颞颌关节关节盘再造的研究

目的：用组织工程方法进行颞颌关节关节盘的再造。方法：用Ⅱ型胶原酶消化法收集兔耳廓软骨细胞，将细胞按5×10^7／ml的终浓度接种于纤维蛋白原中，固化成为膜片状后，将负载软骨细胞的纤维蛋白植入裸鼠背部皮下组织中，2个月后取材，通过大体标本观察、组织学观察颞颌关节关节盘的构建情况。结果：植入术后2个月，在裸鼠背部形成了膜片状的组织，为亮白色，具有一定的弹性和韧性；组织学检查见新形成的组织由软骨构成，纤维蛋白己完全吸收。结论：纤维蛋白负载软骨细胞，可以有效进行颞颌关节关节盘的再造。

Experimental research on the fabrication of TMJ disc employing the method of tissue engineering

Objective： In this experiment, fabrication of TMJ disc was conducted by seeding chondrocytes into fibrin glue. Method： Rabbit chondrocytes were harvested by collagenase type 11 digestion and seeded into fibrin glue at the final density of 5 × 10^7 / ml.The cell- fibrin glue complexes were incubated in vitro for 1 week before implanted subcutaneously into nude mice back. The fabrication of TMJ disc was observed by gross inspection and histological examination 2 months after implantation. Result： New tissue in the shape of TMJ disc was restored successfully 2 months after implantation. The specimens showed the appearance of glistering white and had good flexibility. And histological examination demonstrated that newly restored tissue was constituted of cartilage. Conclusion： It was an efficient method to fabricate TMJ disc by seeding chondrocytes into fibrin glue.