动脉粥样硬化性血栓性脑梗死与HLA-DQA1肽结合基序的相关性研究

目的：针对动脉粥样硬化性血栓性脑梗死（atherothrombotic brain infarction,ABI)多基因致病特点，探讨ABI患者的分子免疫遗传学背景。方法：采用聚合酶链反应－序列特异性引物技术检测了81例ABI患者及99名正常对照的HLA－DQA1位点的等位基因频率。结果：（1）HLA－DQA1＊0301等位基因频率明显升高（χ^2=10.9610,P＜0．001），差异有显著性；（2）有高血压家族史的ABI患者较无高血压家族史的ABI患者HLA－DQA1＊0301等位基因明显升高（χ^2=6.7472,P＜0．01),差异有显著性；（3）ABI患者的HLA－DQA1＊0103等位基因频率较正常对照明显降低（χ^2=5.6313,P＜0．05），差异有显著性。结论：HLA－DQA1＊0301等位基因与ABI的发病有一定的关系，与有原发性高血压阳性家庭史的ABI的发病可能有关，而HLA－DQA1＊0103等位基因可能是ABI的保护性基因。

A study of association between atherothrombotic brain infarction and HLA-DQA1 peptide binding motifs

OBJECTIVE: To analyze the hereditary susceptibility for HLA-DQA1 alleles according to trait of multiple genes leading to disease in atherothrombotic brain infarction (ABI). METHODS: HLA-DQA1 alleles in ABI patients (n=81) and healthy controls (n=99) were detected by PCR-SSP techniques. RESULTS: (1)The frequency of HLA-DQA1*0301 was significantly higher in ABI patients. (2)The frequency of HLA-DQA1*0301 was significantly higher in patients with family history of essential hypertension (EH). (3)The frequency of HLA-DQA1*0103 was significantly lower in ABI patients. CONCLUSION: HLA-DQA1*0301 allele might be a correlative gene with hereditary susceptibility of ABI and HLA-DQA1*0301 allele might be a correlative gene in ABI patients with the family history of essential hypertension, whereas HLA-DQA1*0103 allele might be a protective gene in the patients with ABI.