| 绞股蓝总甙对大鼠心、脑微粒体Na~＋，K~＋－ATP酶活性的抑制作用 |
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| 引用本文： | 韩晓燕,卫洪波,王幼林.绞股蓝总甙对大鼠心、脑微粒体Na~＋，K~＋－ATP酶活性的抑制作用[J].河南医学研究,1995(3). |
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| 作者姓名： | 韩晓燕 卫洪波 王幼林 |
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| 作者单位： | 洛阳医学高等专科学校药理学教研室，南京医学院药理教研室 |
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| 摘 要： | 本文对照人参总甙观察了绞股蓝总甙对大鼠心、脑微粒体Ｎａ￣＋，Ｋ￣＋－ＡＴＰ酶活性的影响。结果表明：绞股蓝总甙体外可逆性抑制鼠心、脑微粒体ＡＴＰ酶，并具有浓度依赖性，ＩＣ＿（５０）分别为５８．７９±８．０５ｍｇ／Ｌ和５２．０７±６．２５ｍｇ／Ｌ。Ｎａ￣＋，Ｋ￣＋对绞股蓝总甙的抑酶作用有拮抗。酶动力学分析显示：绞股蓝总甙对微粒体Ｎａ￣＋，Ｋ￣＋－ＡＴＰ酶的底物ＡＴＰ的抑制为反竞争性抑制。结果提示：绞股蓝总甙的强心和中枢抑制作用可能与对心、脑Ｎａ￣＋，Ｋ￣＋－ＡＴＰ酶的抑制作用有关。
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| 关 键 词： | 人参总甙，绞股蓝总甙，钠、钾－腺苷三磷酸酶，心肌，脑，微粒体 |
INHIBITORY EFFECTS OF GYPENOSIDE ON RAT HEART AND BRAIN Na￣+,K￣+-ATPase
ACTIVITY |
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| Han Xiao-yan Wei Hong-buo Wang You-lin.INHIBITORY EFFECTS OF GYPENOSIDE ON RAT HEART AND BRAIN Na￣+,K￣+-ATPase
ACTIVITY[J].Henan Medical Research,1995(3). |
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| Authors: | Han Xiao-yan Wei Hong-buo Wang You-lin |
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| Abstract: | Comparing with
ginsenoside (Gin ). the effects of gypenoside(Gyp)on themicrosomal Na￣+,K￣+-ATPase from
rat hearts and brains were investigated.The resultsshowed that Gyp inhibited the enzyme
rapidly and reversibly in vitro. The inhibition was in aconcentration- dependent manner. The
IC_(50) of Gyp for the heart and brain was 58.79 + 8.05and 52.07±6.25 mg/L,respectively.The
inhibition was enhanced with increasing the con-centration of ATP or decreasing that of Na￣+
and K￣+. Kinetic analysis revealed that Gyp wasan uncompetitive inhibitor with respect to ATP.
These results suggested that the positive in-otropic effect and the inhibition of CNS by Gyp are
related to its inhibition on the activity ofNa￣+,K￣+-ATPase from hearts or brains. |
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| Keywords: | ginsenoside gypenoside sodium potassium adenosine triphosphatase heart hrain microsomes |
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