当归注射液对庆大霉素耳中毒影响的实验研究

目的以脑干听觉诱发电位(BAEP)、酸性磷酸酶(ACP)组织化学检测为指标,研究当归注射液对庆大霉素(GE)耳毒性的防治作用。方法将30只耳廓反射正常的健康杂色豚鼠随机分成正常对照组、GE组、当归组,各组连续用药20天。结果当归组BAEP阈值升高幅度、波峰潜伏期与波间期变化明显低于GE组(P<0.01),ACP染色变化和毛细胞损害程度均低于GE组(P<0.01)。结论当归注射液能降低GE的耳毒性。保护耳蜗毛细胞内溶酶体的完整性,防止毛细胞的自溶性损伤可能是当归的作用机制之一。

Effects of Angelica injection on gentamycin ototoxicity

Objective:The protective effect of Angelica injection on ototoxicity caused by gentamycin(GE)was studied by means of brainstem auditory evoked potential(BAEP)and histochemical detection of acid phosphatase(ACP) in the guinea pigs.Methods: Thirty healty mottle guinea pigs with normal auricle reflex were randomly divided into three groups: control group;GE group;Angelica group.all animals were administered for 20 days.Results: In the Angelica group,not only the increase amplitude of BAEP threshold but also the prolongation of its wave latencies and interpeak latencies were significantly lower than those in the GE group(p<0.01);the change of ACP development and injury degree of hairy cells were lower than those in the GE group(p<0.01).Conclusion: Angelica injection can reduce GE ototoxicity.Protect integrity of lysosome in hairy cells of the cochlea,and prevent from autolysis of the hair cells,which may be one of the action mechanisms.