大肠癌组织胃泌素表达与PCNA、AgNORs的关系

目的研究大肠癌组织胃泌素表达与增殖细胞核抗原(PCNA)及核仁组成区嗜银染色(AgNORs)的关系.方法采用免疫组化和改良银染法检测48例大肠癌患者癌组织及癌旁粘膜胃泌素和PCNA的表达及AgNORs计数.结果大肠癌组织胃泌素阳性率为39.58%,高分化腺癌明显高于低分化和粘液腺癌(P<0.05).癌组织的PCNA-LI及AgNORs计数显著高于癌旁粘膜和正常粘膜(P<0.01),胃泌素阳性表达组癌组织PCNA-LI和AgNORs计数均明显高于胃泌素阴性表达组(P<0.01).结论部分大肠癌细胞可通过自分泌方式合成产生胃泌素,促进癌细胞的增殖.

Relationship between the expression of gastrin and PCNA,AgNORs in colorectal cancer tissue

Objectives To study the relationship between gastrin expression and proliferating cell nuclear antigen (PCNA),argyrophilia nucleolar organizer regions(AgNORs) in colorectal cancer (CC) tissue. Methods Gastrin and PCNA expression in 48 cases of CC tissue and cancer adjacent mucosa was detected by immunohistochemistry techniques. AgNORs was determined with argyrophilia stain. Results The positive rate of gastrin in CC tissue was 39 58%, that of well differentiated adenocarcinoma was higher than the poorly differentiated and mucinous adenocarcinoma( P <0 05).The PCNA LI and AgNORs count in CC tissue was higher than that in cancer adjacent and normal mucosa ( P <0 01).The PCNA LI and AgNORs count in the group of gastrin positive expression was higher than in gastrin negative group. Conclusions Some CC cells synthesize gastrin and in turn stimulate the proliferation of cancer cells.