| 用生物测定法研究肉苁蓉多糖在体外的吸收转运 |
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| 引用本文： | 齐云,蔡润兰,王翔岩,李蒙,李晓红,杨美华. 用生物测定法研究肉苁蓉多糖在体外的吸收转运[J]. 中国药学杂志, 2010, 45(7): 527-530 |
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| 作者姓名： | 齐云 蔡润兰 王翔岩 李蒙 李晓红 杨美华 |
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| 作者单位： | 中国医学科学院-北京协和医院药用植物研究所; |
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| 基金项目： | 国家自然科学基金资助项目(30672659); 宁夏回族自治区科技攻关计划项目(05GG-10807) |
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| 摘 要： | 目的 用生物测定法考察肉苁蓉多糖（CDPS）在Caco-2细胞模型的吸收转运。方法 用培养于Transwell上的Caco-2细胞模型研究CDPS的吸收转运；采用生物活性测定法测定CDPS在接受侧介质中的含量。结果 Caco-2细胞单层极性与完整性符合实验要求，CDPS在1.87 ~ 40 mg·L-1内，与RAW264.7细胞NO分泌呈明显量效关系，转运量随供侧（AP侧）浓度的增加而增大，不同浓度CDPS Papp( A-B)均小于1 × 10-7 cm·s-1。结论 采用生物活性测定CDPS的Papp具可行性，该药属吸收不良药物，口服吸收率应小于1％。
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| 关 键 词： | 肉苁蓉多糖 Caco-2细胞单层模型 |
| 收稿时间： | 2012-01-01; |
Absorption and Transportation of Cistanche deserticola Polysaccharides Using Bioactivity Assay in Vitro |
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| QI Yun,CAI Run-lan,WANG Xiang-yan,LI Meng,LI Xiao-hong,YANG Mei-hua. Absorption and Transportation of Cistanche deserticola Polysaccharides Using Bioactivity Assay in Vitro[J]. Chinese Pharmaceutical Journal, 2010, 45(7): 527-530 |
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| Authors: | QI Yun CAI Run-lan WANG Xiang-yan LI Meng LI Xiao-hong YANG Mei-hua |
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| Affiliation: | QI Yun,CAI Run-lan,WANG Xiang-yan,LI Meng,LI Xiao-hong,YANG Mei-hua(Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193,China) |
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| Abstract: | OBJECTIVE To study the absorption and transportation of Cistanche deserticola polysaccharides(CDPS)in Caco-2 monolayer model by utilizing bioactivity assay.METHODS The transwell filter inserts on culturing Caco-2 cell monolayer was used to observe the absorption and transportation of CDPS.Bioactive assay approach was used to determine the content of CDPS in the receiver compartment.RESULTS The integrity and polarity of Caco-2 cell monolayer were conformed to the experiment requirements.CDPS in the concentra... |
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| Keywords: | Cistanche deserticola polysaccharides(CDPS) Caco-2 cell monolayer model apparent permeability coefficient(Papp) absorption transport |
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