白藜芦醇诱导K562/AO_2凋亡与mdr1基因表达的关系

目的研究白藜芦醇(Res)体外对K562/AO2增殖及凋亡的影响,并探讨与耐药基因mdr1表达的关系。方法用噻唑蓝比色法(MTT)检测对照组、不同剂量Res组(25~200μmol/L)作用48 h后K562/AO2增殖率,用流式细胞仪检测各组凋亡率,并用逆转录-聚合链反应(RT-PCR)法检测各组mdr1 mRNA的表达。结果Res体外对人K562/AO2细胞有显著增殖抑制及凋亡诱导作用,Res组(25μmol/L、50μmol/L、100μmol/L、200μmol/L)作用48 h后其凋亡率均显著高于对照组(P<0.05);mdr1 mRNA表达相对强度均显著低于对照组(P<0.05),且随浓度增加而表达减弱。结论Res体外能诱导K562/AO2细胞的凋亡,且呈一定的浓度依赖性,其作用机制可能与逆转mdr1 mRNA基因表达有关。

The effects of resveratrol on apoptosis of K562/AO_2 and its relationship with mdr1 mRNA

Objective It is to study the effects of resveratrol (Res) on apoptosis and proliferation of K562/AO2 in vitro,and to explore its relationship with expression of mdr1 mRNA.Methods Methyl thiazolyl tetrazolium method(MTT) and flowcytometry were used to detect the cell proliferation rate and apoptosis rate of K562/AO2 in control group and groups treated with Res of different dosage(25-200 μmol/L).The expression of mdr1 mRNA was detected by RT-PCR.Results Res had a significant inhibiting proliferation and inducing function on human K562/AO2 cells in vitro.Apoptosis rates in Res groups(25 μmol/L,50 μmol/L,100 μmol/L,200 μmol/L) were significantly higher than that in control groups(P<0.05).The relative intensities of expression of mdr1 mRNA,which had a negative relationship with concentration of Res were significantly lower than that in control group.Conclusion Res may inhibt the proliferation and induce apoptosis of K562/AO2 cells in vitro in concentration-dependent,the mechanisms of which may be related to the down regulation of expression of mdr1 mRNA.