| 氯化钾除极对分离的豚鼠心室肌细胞胞浆内[Ca^2＋]i的影响 |
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| 引用本文： | 董德利,沃雪.氯化钾除极对分离的豚鼠心室肌细胞胞浆内[Ca^2＋]i的影响[J].哈尔滨医科大学学报,1999,33(1):9-11. |
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| 作者姓名： | 董德利 沃雪 |
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| 作者单位： | [1]药理学教研室 [2]二院药学部 |
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| 基金项目： | 卫生部优秀青年科学基金 |
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| 摘 要： | 用酶解法分离单个豚鼠心室肌细胞，以荧光探针Ｆｌｕｏ－３／ＡＭ标记胸浆内游离钙离子，用激光扫人聚焦显微镜实时测定胞浆内「Ｃａ＾＋」变化。分别观察了在正常台氏液和无钙台氏液中３０ｍｍｏｌ／ＬＫＣｌ除极对胞浆内「Ｃａ＾２＋」ｉ的影响。
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| 关 键 词： | 氯化钾 Fluo－3／AM 心室肌细胞 游离钙 |
Effect of Depolarization by KCl on Cytosolic [Ca 2 ] i of Isolated Cardiac Ventricular Myocytes of Guinea Pig |
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| Dong Deli et al.Effect of Depolarization by KCl on Cytosolic [Ca 2 ] i of Isolated Cardiac Ventricular Myocytes of Guinea Pig[J].Journal of Harbin Medical University,1999,33(1):9-11. |
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| Authors: | Dong Deli |
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| Institution: | Department of Pharmacology |
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| Abstract: | To investigate the relationship between the membrane depolarization and the release of intracellular Ca 2 stores, single cardiac ventricular myocytes of guinea pig were isolated by an enzymatic technique and loaded with the Ca 2 indicator Fluo 3/AM. The changes of cytosolic Ca 2 ] i of ventricular myocytes depolarized by 30mmol/L KCl in normal Tyrode solution and Ca 2 free Tyrode solution were observed by using laser scanning confocal microscope. Results showed that depolarization by 30mmol/L KCl caused constant increase of cytosolic Ca 2 ] i during 250s in normal Tyrode solution and transient increase in Ca 2 free Tyrode solution. We concluded that depolarization caused the Ca 2 release from intracellular Ca 2 stores and Ca 2 influx was not required to mediate, the constant increase of cytosolic Ca 2 ] i was sustained by Ca 2 influx. |
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| Keywords: | KCl Fluo 3/AM Cytosolic [Ca 2 ] i Depolarization |
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