The in vitro biosynthesis of steroids by the gonad of the mussel (Mytilus edulis) |
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Authors: | D de Longcamp P Lubet M Drosdowsky |
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Institution: | 1. Départment de Biochimie, Faculté de Médecine, Université de Caen, France;2. Départment de Zoologie, Faculté des Sciences, Univesité de Caen, France |
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Abstract: | In vitro steroid biosynthesis in the male and female gonad of Mytilus edulis was investigated. Using tritium and 14C-labeled precursors, four enzyme systems were demonstrated: a 3β-hydroxysteroid dehydrogenase-Δ5,4-isomerase comples (pregnenolone→progesterone; 17α-hydroxypregnenolone→17α-hydroxyprogesterone; dehydroepiandrosterone→androstenedione), a C17,20-lyase (17α-hydroxypregnenolone→dehydroepiandrosterone; 17α-hydroxyprogesterone→androstenedione), a 17β-hydroxysteroid dehydrogenase (dehydroepiandrosterone→androstenediol; androstenedioneαtestosterone; estradiol-17βαestrone) and a 5α-reductase (testosterone→5α-dihydrotestosterone). The yields were very low (0.2–1%) except when estrogens were used (10–50%). Radioimmunoassay techniques indicate the presence of testosterone and possibly estrogens in the gonad of the mussel at two different stages in its gametogenic cycle. |
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