通窍鼻炎颗粒质量标准研究

目的:建立通窍鼻炎颗粒中欧前胡素和黄芪甲苷的含量测定方法.方法:采用高效液相色谱法.以迪马Diamonsil C18(4.6 mm×250 mm,5μm)色谱柱,流动相甲醇-水(54:46),检测波长248 nm,测定欧前胡素的含量;以菲罗门100A C18(4.6 mm×150 mm,5μm)色谱柱,蒸发光散射检测器,乙腈-水(32:68)为流动相测定黄芪甲苷的含量.结果:欧前胡素在0.072 5～0.773 6μg线性关系良好,黄芪甲苷在1.462 8 ～7.314 μg线性关系良好,其精密度、重复性、稳定性均符合要求.结论:方法准确可靠,可用于通窍鼻炎颗粒的质量控制.

Study on Quality Standand of Tongqiaobiyan Granule

Objective:To establish the methods for determination of imperatorin and astragaloside in Tongqiaobiyan granule.Method:The HPLC method was performed on Diamonsil C18(4.6 mm×250 mm,5 μm)column;a mixture liquid of methanol-water(54∶46) was used as the mobile phase,and the detective wavelength was at 238 nm to determine the content of imperatorin.With Philo door 100A C18(4.6 mm×150 mm,5 μm)column and evaporative light scattering detector,a mixture liquid of acetonitrile-water(32∶68)was used as the mobile phase to determine the content of astragaloside.Result:The calibration curve of imperatorin and astragaloside were linear in the ranges of 0.072 5-0.773 6,1.462 8-7.314 μg respectively.Conclusion:It is an accurate and credible quality control method.