金银花-连翘药对中绿原酸和连翘苷在大鼠各组织中的分布研究

目的:通过绿原酸和连翘苷在大鼠各组织中的含量测定,分析金银花-连翘药对的作用特点.方法:采用HPLC,依利特Betasil C18(4.6 mmx200 mm,5μm)色谱柱,乙腈-0.4％磷酸溶液(30∶ 70)为流动相,检测波长280 nm,流速1.0 mL·min-1的色谱条件分析绿原酸和连翘苷在大鼠各组织中的含量.结果:各组织中均检测到绿原酸和连翘苷,除消化道外,脑中含量最高.结论:各组织中的绿原酸和连翘苷含量分布符合金银花连翘药对“皆轻清宣散,偏于清透上半身之邪”的特点.

Determination of Chlorogenic acid and Frusthin of the Drug Pairs of Flos Lonicerae and Fructus Forsythiae in Rats'Organs

Objective:To analyze the feature of the drug pairs of Flos Lonicerae and Fructus Forsythiae by determining the content of chlorogenic acid and forsythin.Method: The content of chlorogenic acid and forsythin in different organs was analyzed by using the Diamonsil C18(4.6 mm×200 mm,5 μm) column with acetonitrile-0.4% phosphoric acid as mobile phase;the velocity of flow was 1.0 mL · min-1,and the detection wavelength was set at 280 nm.Result: Chlorogenic acid and forsythin can be detected in all the organs and there was more in brain except the gastrointestinals.Conclusion: The distribution of chlorogenic acid and forsythin fit in with the feature’clearing away the heat-evil to relieve the exterior with light drug’.