| 正交试验优选茶梗中茶氨酸的提取工艺 |
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| 引用本文： | 陈沛鑫,高英,李卫民.正交试验优选茶梗中茶氨酸的提取工艺[J].中国实验方剂学杂志,2012,18(17):39-41. |
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| 作者姓名： | 陈沛鑫 高英 李卫民 |
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| 作者单位： | 1. 广州中医药大学中药学院,广州,510006
2. 广州中医药大学新药研究开发中心,广州,510006 |
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| 摘 要： | 目的:优选茶梗中茶氨酸的提取工艺.方法:以茶氨酸为指标,HPLC测定茶氨酸含量,选择提取溶剂、料液比、提取温度、提取次数和提取时间为考察因素,用单因素试验确定因素水平;以料液比、提取温度、提取时间为考察因素,采用正交试验优选提取工艺.结果:最佳提取工艺为20倍量水于80℃提取1h.结论:该优选工艺稳定、可控、提取率高.
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| 关 键 词： | 茶梗 茶氨酸 单因素考察 正交试验 |
| 收稿时间： | 4/8/2012 12:00:00 AM |
Optimization of Extraction Technology of Theanine from Tea Stalk by Orthogonal Test |
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| CHEN Pei-xin,GAO Ying and LI Wei-min.Optimization of Extraction Technology of Theanine from Tea Stalk by Orthogonal Test[J].China Journal of Experimental Traditional Medical Formulae,2012,18(17):39-41. |
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| Authors: | CHEN Pei-xin GAO Ying and LI Wei-min |
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| Institution: | School of Chinese Materia Medica,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;New Drug Research and Development Center,Guangzhou University of Chinese Medicine, Guangzhou 510006,China;School of Chinese Materia Medica,Guangzhou University of Chinese Medicine,Guangzhou 510006,China |
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| Abstract: | Objective:To optimize extraction technology of theanine from tea stalk.Method:With the content of theanine as index which was determined by HPLC,ratio of liquid-material,extraction solvent,extraction temperature,extraction time and extraction times were selected as factors,factors and levels were determined by single-factor test;Orthogonal test was used to optimize extraction technology with ratio of liquid-material,extraction temperature and extraction time as factors.Result:Optimum extraction process was:extracted 1 h with 20 times the amount of water at 80 ℃.Conclusion:This optimized technology was stable and reliable with high extraction rate. |
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| Keywords: | tea stalk theanine single-factor test orthogonal test |
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