蒲黄总黄酮对家兔血液流变学参数和血小板聚集的影响

目的:研究蒲黄总黄酮对血液流变学参数和血小板聚集的影响.方法:将雌性家兔48只随机分为正常组(等容量生理盐水)、模型组(等容量生理盐水)、丹参组(含丹参酮0.13 g·kg-1)、蒲黄总黄酮高、中、低剂量组(0.8,0.5,0.2g·kg-1).除正常对照组sc生理盐水外,其余各组sc肾上腺素加冰水冷浴制造血瘀证模型,观察蒲黄总黄酮对急性血瘀证家兔血液流变性的影响;采用家兔血小板聚集试验,研究蒲黄总黄酮抗血栓形成的作用.结果:蒲黄总黄酮各剂量均可降低急性血瘀模型家兔全血各切变率黏度,减小红细胞压积,差异有统计学意义(P＜0.05或P＜0.01);且可明显降低血小板最大聚集率,缩短最大凝集时间,差异有统计学意义(P＜0.01).结论:蒲黄总黄酮可显著改善急性血瘀证家兔血液流变性,同时具有抑制血小板聚集的作用.

Effect of Pollen Typhae Flavonoids on Hemorheological Parameters and Platelet Aggregation in Rabbits

Objective:To study the effect of pollen typhae flavonoids on hemorheological parameters and platelet aggregation.Method: Forty-eight female rabbits were divided into normal group(saline),model group(saline),Salvia miltiorrhiza group(tanshinone 0.13 g · kg-1),pollen typhae flavonoids high,median,low dose group(dose of 0.8,0.5,0.2 g · kg-1).Except for the normal group which was given sc saline,other groups were given sc adrenaline hydrochloride and socked in ice-water,so as to establish acute blood stasis model.Then the effect of pollen typhae flavonoids on hemorheological parameters was studied by this model.Based on the platelet aggregation in rabbits,the inhibitory action of pollen typhae flavonoids on the formation of thrombosis was studied.Result: Pollen typhae flavonoids could significantly reduce viscosities at each shear rates and HCT for blood stasis model,there were significant differences(P<0.05 or P<0.01),and could significantly reduce the maximum platelet aggregation rate and shorten the maximum agglutinating time with significant differences(P<0.01).Conclusion: Pollen typhae flavonoids can obviously ameliorate the hemorheological parameters and inhibit the platelet aggregation.