| HPLC测定舞草中木犀草素的含量 |
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| 引用本文： | 潘力,廖厚知.HPLC测定舞草中木犀草素的含量[J].中国实验方剂学杂志,2012,18(17):145-147. |
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| 作者姓名： | 潘力 廖厚知 |
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| 作者单位： | 1. 广西柳州市红十字医院药剂科,广西柳州,545000
2. 广西汇科药物研究所,南宁,530007 |
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| 摘 要： | 目的:建立舞草中木犀草素的含量测定方法.方法:采用VP-ODS C18(4.6 mmx250 mm,5μm)色谱柱.以甲醇-0.3％磷酸溶液(60:40)为流动相,流速1.0 mL· min-1,检测波长350 nm.结果:舞草中的木犀草素含量测定方法在0.0407 2 ～0.6515 2进样量与峰面积的线性关系良好(r=0.999 8);平均回收率100.35％,RSD 0.80％.结论:本方法准确、灵敏、重复性好,可用于舞草的质量控制.
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| 关 键 词： | 舞草 木犀草素 高效液相色谱法 |
| 收稿时间： | 2012/3/30 0:00:00 |
Determination of Luteolin in Codariocalyx motorius by HPLC |
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| PAN Li and LIAO Hou-zhi.Determination of Luteolin in Codariocalyx motorius by HPLC[J].China Journal of Experimental Traditional Medical Formulae,2012,18(17):145-147. |
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| Authors: | PAN Li and LIAO Hou-zhi |
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| Institution: | Department of Pharmacy, Red Cross Hospital of Liuzhou, Liuzhou 545000,China;Guangxi Huike Institute of Materia Medica, Nanning 530007,China |
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| Abstract: | Objective:To establish the determination method of luteolin content in Codariocalyx motorius.Method:VP-ODS Cl8(4.6 mm×250 mm,5 μm) colmnn was used,the mobile phase was methanol-0.3% phosphonic acid solution(60∶40) with the flow rate as 1.0 mL · min-1.The detection wavelength was set at 350 nm.Result:The linear relation of hyperin was good in the range from 0.040 72 to 0.651 52 μg.The average recovery was 100.35%,RSD was 0.80%.Conclusion:The method established is accurate,sensitive and good in reproducibility.It can be applied to the quality control of C.motorius. |
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| Keywords: | Codariocalyx motorius luteolin HPLC |
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