| 聚酰胺树脂精制绞股蓝总黄酮的工艺优选 |
| |
| 引用本文： | 罗朵生,朴胜华,黄利华,郭姣.聚酰胺树脂精制绞股蓝总黄酮的工艺优选[J].中国实验方剂学杂志,2012,18(17):17-19. |
| |
| 作者姓名： | 罗朵生 朴胜华 黄利华 郭姣 |
| |
| 作者单位： | 1. 国家中医药管理局高脂血症“调肝降脂”重点研究室,国家中医药管理局“脂代谢”三级实验室,广东药学院,广州 510006
2. 广东省代谢性疾病中医药防治重点实验室,广州中医药大学,广州 510006 |
| |
| 基金项目： | 广州市科技计划项目(2009Z1-E361);广东省自然科学基金团队项目(10351022401000000);广东省教育部产学研结合项目(2009B090300349) |
| |
| 摘 要： | 目的:优选聚酰胺树脂分离纯化绞股蓝总黄酮的工艺条件.方法:采用紫外分光光度法,以总黄酮含量为考察指标,研究在静态和动态条件下,聚酰胺树脂吸附和洗脱总黄酮的行为,筛选出聚酰胺树脂精制绞股蓝总黄酮的最优工艺参数.结果:最佳工艺参数为生药质量浓度0.35 g·mL-1,上样液pH 3,温度为室温,4 BV 60％乙醇洗脱.此工艺条件下,总黄酮纯度由粗品的23.91％提高到81.97％,纯化物中总黄酮保留率高达90.11％.结论:采用聚酰胺树脂纯化绞股蓝总黄酮效果良好,适用于工业化生产.
|
| 关 键 词： | 绞股蓝 总黄酮 聚酰胺树脂 精制 |
| 收稿时间： | 3/8/2012 12:00:00 AM |
Optimization of Purification Technology for Total Flavonoids from Gynostemma pentaphyllum by Polyamide Resin |
| |
| LUO Duo-sheng,PIAO Sheng-hu,HUANG Li-hua and GUO Jiao.Optimization of Purification Technology for Total Flavonoids from Gynostemma pentaphyllum by Polyamide Resin[J].China Journal of Experimental Traditional Medical Formulae,2012,18(17):17-19. |
| |
| Authors: | LUO Duo-sheng PIAO Sheng-hu HUANG Li-hua and GUO Jiao |
| |
| Institution: | Key Unit of Modulating Liver to Treat Hyperlipemia,State Administration of Traditional Chinese Medicine(SATCM),Level 3 Laboratory of Lipid Metabolism SATCM,Guangdong Pharmaceutical University,Guangzhou 510006,China;Key Unit of Modulating Liver to Treat Hyperlipemia,State Administration of Traditional Chinese Medicine(SATCM),Level 3 Laboratory of Lipid Metabolism SATCM,Guangdong Pharmaceutical University,Guangzhou 510006,China;Key Unit of Modulating Liver to Treat Hyperlipemia,State Administration of Traditional Chinese Medicine(SATCM),Level 3 Laboratory of Lipid Metabolism SATCM,Guangdong Pharmaceutical University,Guangzhou 510006,China;Guangdong TCM Key Laboratory for Metabolic Diseases, Guangzhou University of Chinese Medicine,Guangzhou 510006,China |
| |
| Abstract: | Objective: To optimize purification technology for total flavonoids from Gynostemma pentaphyllum by polyamide resin. Method: With the content of total flavonoids as index,UV spectrophotometry was used, adsorption and elution behavior of polyamide resin for total flavonoids was investigated under static and dynamic conditions, and to select optimum technology parameters of purification technology for total flavonoids by polyamide resin. Result: Optimum purification technology parameters were:at room temperature,the concentration of crude drug 0.35 g·mL-1,pH 3.0 and 4 BV 60% ethanol as eluant. Under these conditions,purity of total flavonoids increased from 23.91% to 81.97%,retention rate of total flavonoids in the purified was up to 90.11%. Conclusion: Purified effect of total flavonoids from G. pentaphyllum by polyamide resin was preferable,it was suitable for industrial production. |
| |
| Keywords: | Gynostemma pentaphyllum total flavonoids polyamide resin purification |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国实验方剂学杂志》浏览原始摘要信息 |
| 点击此处可从《中国实验方剂学杂志》下载免费的PDF全文 |
|
