| 高效液相色谱测定莱菔子总碱中芥子碱硫氰酸盐含量 |
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| 引用本文： | 蒋海强,丁臻,周洪雷. 高效液相色谱测定莱菔子总碱中芥子碱硫氰酸盐含量[J]. 中国实验方剂学杂志, 2012, 18(17): 78-80 |
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| 作者姓名： | 蒋海强 丁臻 周洪雷 |
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| 作者单位： | 1. 山东中医药大学,山东济南,250355
2. 安徽省亳州市人民医院,安徽亳州,236800 |
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| 基金项目： | 国家自然科学基金项目(81072794/H2708);山东省科技发展计划项目(2008GG2NS02011);国家科技重大专项(2009ZX09301-013);山东省自然科学基金项目(ZR2010HM017);山东省教育厅科技计划项目(J105LL05) |
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| 摘 要： | 目的:建立莱菔子总碱中芥子碱硫氰酸盐的含量测定方法.方法:采用高效液相色谱法,以乙腈-0.08 mol·L-1KH2P04溶液(12:88)为流动相,流速1.0 mL·min-1进行检测.结果:芥子碱硫氰酸盐线性范围为0.482 ～2.892 μg,平均加样回收率103.63％.结论:该法快速、简便,结果准确、可靠,可用于莱菔子总碱中芥子碱硫氰酸盐的测定.
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| 关 键 词： | 莱菔子总碱 芥子碱硫氰酸盐 高效液相 |
| 收稿时间： | 2011-10-18 |
Determination of Sinapine Cyanide Sulfonate in Semen Raphani Total Alkaloids by RP-HPLC |
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| JIANG Hai-qiang,DING Zhen and ZHOU Hong-lei. Determination of Sinapine Cyanide Sulfonate in Semen Raphani Total Alkaloids by RP-HPLC[J]. China Journal of Experimental Traditional Medical Formulae, 2012, 18(17): 78-80 |
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| Authors: | JIANG Hai-qiang DING Zhen ZHOU Hong-lei |
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| Affiliation: | Shandong University of Traditional Chinese Medicine, Ji'nan 250355, China;People's Hospital of Bozhou City, Bozhou 236800, China;Shandong University of Traditional Chinese Medicine, Ji'nan 250355, China |
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| Abstract: | Objective: To establish the method for content determination of sinapine cyanide sulfonate in Semen Raphani total alkaloids.Method: The determination was carried out with HPLC,acetonitrile-H2O-0.08 mol · L-1 KH2P04(12∶88) as the mobile phase at a flow rate 1.0 mL · min-1.Result:The linear range of Sinapine cyanide sulfonate was 0.482-2.892 μg and the average recovery was 103.63%.Conclution:The method is rapid,simple,accurate and reliable in results,and suitable for the determination of sinapine cyanide sulfonate in Semen Raphani total alkaloids. |
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| Keywords: | Semen Raphani total alkaloids sinapine cyanide sulfonate HPLC |
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