荧光凝胶色谱法测定大鼠单次口服麦冬多糖MDG-1排泄变化

目的:探讨单次口服麦冬多糖MDG-1后排泄量的变化.方法:采用异硫氰酸荧光素(FITC)对麦冬多糖MDG-1进行标记( F-MDG-1),测定取代度.采用高效凝胶色谱法(HPGPC)对粪便及尿液内MDG-1含量进行测定.SD-雄性大鼠,给药组按照300 mg· kg-给予F-MDG-1,空白组给予纯水,分别在0,4,12,24,48,72 h收集尿液及粪便.测定含量.结果:在单次给予F-MDG-1量300 mg· kg-1后,尿液中含量测定在12 h时达到最大排泄量0.808 7 mg,而后逐渐减少,至72 h最低0.105 3 mg;在粪便测定中在12h达到最大排泄量为21.332 4 mg,而后逐渐减少,至72 h最低0.506 5 mg.结论:采用FITC对MDG-1进行标记,并采用荧光色谱法对MDG-1在排泄物内的含量变化进行研究是可行的.MDG-1基本不被人体吸收,其主要经粪便排泄.

Determination of Contents after single Oral Ophiopogonis Radix Polysaccharides MDG-1 in Rat Urine and Faeces by HPGPC

Objective:To investigate the contents after single oral one kind of homogeneous molecular weight polysaccharide(MDG-1) extracted from Ophiopogonis Radix in rat urine and faeces by high performance gel permeation chromatography(HPGPC).Method: Labeling the MDG-1(F-MDG-1) and detecting replacement of fluorescein isothiocyanate(FITC) then the HPGPC was used to detect the contents of MDG-1 in urine and faeces after single oral administration.The rats were given F-MDG-1 at doses of 300 mg · kg-1 then urine and faeces were collected at predefined time points(0,4,12,24,48,72 h postdose;n=six per time point) then stored at-20 ℃ to be analyzed.Result: The F-MDG-1 in rats urine was described with the max 0.808 7 mg at 12 h and a degradation model to the lowest 0.1053 mg at 72 h.The F-MDG-1 in rats faeces was described with the max 21.332 4 mg in 12 h and a degradation model to the lowest 0.506 5 mg at 72 h.Conclusion:The combination of the FITC prelabeling method with the HPGPC is confirmed and tested and feasibile.This methodology should be helpful to other polysaccharides research in rat urine and faeces.In this study,F-MDG-1 was absorbed hardly and the main excretion way is through faeces.