P-糖蛋白方法用于中药药性理论研究的探讨

探讨P-糖蛋白与中药药性理论的相关性与研究现状,更好开展和利用P-糖蛋白方法用于中药药性理论的研究.介绍了中药药性理论的引经药、配伍关系、配伍解毒等与P-糖蛋白关系的最新进展,提出P-糖蛋白方法用于中药药性理论研究的思考.同时,综合分析了用于研究中药药性理论与P-糖蛋白关系的相关研究方法,包括细胞模型法、基因敲除动物模型法、在体肠灌流法、计算机模拟等的技术特点.一些中药成分能影响P-糖蛋白的活性,可从跨膜蛋白的角度研究中药成分在体内过程的相互作用.通过P-糖蛋白理论与方法的应用,可为中药药性理论的研究提供崭新的思路,有利于促进学科交叉渗透,提高中医药传统理论的现代研究与应用水平.

Application of P-glycoprotein Method in Theoretical Study of Nature of Traditional Chinese Medicine

To explore the relations between the P-glycoprotein and the nature of traditional Chinese medicine (TCM), which will be helpful to a better application of the P-glycoprotein in the study on the nature of TCM. Based on the generalization of the latest progresses of the studies on the relation of the P-glycoprotein and the Meridian-guiding herbs, compatibility relations and detoxification by compatibility, we came up with the thoughts of the nature analysis of TCM by P-glycoprotein. We also analyzed the technical characteristics of the study methods of the relationship between the nature of TCM and the P-glycoprotein, including cell model method, in situ perfusion experiment, gene knock-out animal method, and computer simulation. Since some ingredients in the TCM can affect the activity of the P-glycoprotein, by which the interaction of the TCM in vivo can be illustrated from the angle of the transmembrane protein. By the theoretical and methodical application of the P-glycoprotein, new idears for the study of the theoretical study of the nature of the TCM can be worked out, which is good for the mutual osmosis of subjects and the improvement of the application and the modern research of the traditional theory of TCM.