| 瓜蒌子的TLC鉴别和HPLC含量测定 |
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| 引用本文： | 巢志茂,唐春风,张贵峰,孙文.瓜蒌子的TLC鉴别和HPLC含量测定[J].中国实验方剂学杂志,2012,18(17):86-88. |
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| 作者姓名： | 巢志茂 唐春风 张贵峰 孙文 |
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| 作者单位： | 1. 中国中医科学院中药研究所,北京,100700
2. 广西药用植物园,南宁,530023 |
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| 基金项目： | 中医药行业2011年科研专项(201107009) |
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| 摘 要： | 目的:建立常用中药瓜蒌子的定性定量方法.方法:以栝楼仁二醇和α-菠菜甾醇为对照品,建立瓜蒌子的薄层色谱( TLC)鉴别技术;以栝楼仁二醇为对照品,建立高效液相色谱(HPLC)-紫外(PAD)检测的含量测定技术.结果:12份瓜蒌子的不皂化物,均可显示栝楼仁二醇和α-菠菜甾醇的薄层斑点;瓜蒌子中栝楼仁二醇的含量介于0.056 88％ ～0.087 28％.结论:栝楼仁二醇作为瓜蒌子的有效成分,可以作为瓜蒌子质量标准的一个重要指标.建议中药瓜蒌子中栝楼仁二醇的含量不得低于0.05％.
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| 关 键 词： | 瓜蒌子 栝楼仁二醇 薄层色谱 高效液相色谱 栝楼 |
| 收稿时间： | 2012/2/27 0:00:00 |
TLC Identification and HPLC Assay on Trichosanthis Semen |
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| CHAO Zhi-mao,TANG Chun-feng,ZHANG Gui-feng and SUN Wen.TLC Identification and HPLC Assay on Trichosanthis Semen[J].China Journal of Experimental Traditional Medical Formulae,2012,18(17):86-88. |
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| Authors: | CHAO Zhi-mao TANG Chun-feng ZHANG Gui-feng and SUN Wen |
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| Institution: | Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
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| Abstract: | Objective:To establish the methods of qualitation and quantitation about Trichosanthis Semen,one of the commonly used Chinese material medica.Method: The thin-layer chromatography(TLC) method was found with the reference substance both karounidiol and α-spinasterol.The HPLC method was found with the PAD and the reference substance of karounidiol.Result: The unsaponifiable matter from all 12 Trichosanthis Semen samples showed the spots of both karounidiol and α-spinasterol on a TLC plate.The content of karounidiol in all 12 Trichosanthis Semen samples was 0.056 88%-0.087 28%.Conclusion: Karounidiol,one of the active components of Trichosanthis Semen,can be considered as an important index of the quality standard of Trichosanthis Semen.The content of karounidiol was suggested not less than 0.05% in Trichosanthis Semen. |
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| Keywords: | Trichosanthis Semen karounidiol TLC HPLC Trichosanthes kirilowii |
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