抗人p185单克隆抗体的体外直接抗癌效应

目的:对使用“表面表位包埋法”构建成功的抗人肿瘤抗原p185的单克隆抗体(mAb)的抑癌活性进行研究。方法:采用免疫荧光染色法、MTT法、软琼脂培养法及细胞长期增殖法等,测定3株mAb的对高表达p185的乳腺癌细胞及转染p185基因的成纤维细胞的抑制活性,以及mAb对提高癌细胞的化疗药物敏感性的效应。结果:这3株mAb均可特异性地明显抑制癌变细胞的生长,并不同程度上提高癌细胞对化疗药物的敏感性。结论:这几株mAb具有治疗p185高表达肿瘤的应用前景。

Investigation on the anti-cancer activities of anti-p185 monoclonal antibodies in vitro

To study the anti-cancer activity of the three anti-oncoprotein p185 mAbs established by surface epitope masking method so as to provide some basic information for clinical tumor targeting therapy.Methods: Immnune flurorence staining, MTT assay, soft-ar-gar culture and long-term cell proliferation assay detected the suppressive activities of the mAbs against the breast cancer cells overexpressing p185 and p185-transfected fibroblast cells, and the inducing effects of the mAbs on tumors sensitivity to chemotherapy.Results:The 3 mAbs specifically suppressed tumor cell proliferation and enhanced the sensitivity of tumors to chemotherapeutics. Conclusion:These mAbs have the potentials to develop into the clinical tumor targeting therapeutic reagents of the tumors overexpressing p185.