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Quantitative determination of daumone in rat plasma by liquid chromatography-mass spectrometry |
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| Authors: | Noh Keumhan Park Jung Hyun Park Jong Hee Kim Minkyu Jung Mankil Ha Hunjoo Kwon Kwang-Il Lee Hwa Jeong Kang Wonku |
| Affiliation: | a College of Pharmacy, Yeungnam University, Kyoungsan, Kyoungbuk 712-749, South Korea b Division of Life and Pharmaceutical Sciences, the Center for Cell Signaling & Drug Discovery Research and College of Pharmacy, Ewha Womans University, Seoul 120-750, South Korea c Department of Chemistry, Yonsei University, Seoul 120-749, South Korea d Department of Bioinspired Science, Division of Life and Pharmaceutical Sciences, the Center for Cell Signaling & Drug Discovery Research and College of Pharmacy, Ewha Womans University, Seoul 120-750, South Korea e College of Pharmacy, Chungnam National University, Daejeon 305-764, South Korea |
| Abstract: | Daumone, 6-(3,5-dihydroxy-6-methyl-tetrahydro-pyran-2-yloxy)-heptanoic acid is a pheromone secreted by Caenorhabditis elegans, and has been known as a pivotal regulator of chemosensory processes in development and ageing. A quantification method using mass spectrometry was developed for the determination of daumone in rat plasma. After simple protein precipitation with acetonitrile including an internal standard, the analytes were chromatographed on a reversed-phase column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma daumone concentrations after a single intravenous administration of daumone in rats. |
| Keywords: | Daumone Rat plasma LC-MS/MS |
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