Genetic association of blaSHV-5 with transposable elements IS26 and IS5 in Klebsiella pneumoniae from Taiwan |
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| Authors: | W L Yu S C Chen S W Hung Y C Chuang J G Chung I C Chen L T Wu |
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| Institution: | Department of Critical Care Medicine, Chi-Mei Medical Center, Tainan, Taiwan. |
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| Abstract: | A cloned 5,248-bp EcoRI fragment from the Klebsiella pneumoniae transferable plasmid pKP53 (> 70 kb) containing bla(SHV-5) was sequenced. Insertion sequences IS26 and IS5 were found downstream from bla(SHV-5). The DNA sequences of the genetic environment surrounding bla(SHV-5) were homologous to plasmid p1658/97 from Escherichia coli, containing a truncated recF gene and a truncated deoR gene upstream and downstream from bla(SHV-5), respectively. RecF may be involved in bla(SHV-5) translocation to the plasmid by RecF-dependent recombination. This novel genetic environment may be associated with the successful proliferation and/or expression of SHV-5 in K. pneumoniae strains from Taiwan. |
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| Keywords: | bla SHV gene DNA sequence insertion sequence Klebsiella pneumoniae RecF sequencing |
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