| 前列腺癌组织中骨桥蛋白及基质金属蛋白酶-2检测 |
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| 引用本文： | 武玉东,刘秉乾,张雪培,魏金星,吴长利.前列腺癌组织中骨桥蛋白及基质金属蛋白酶-2检测[J].郑州大学学报(医学版),2005,40(4):654-656. |
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| 作者姓名： | 武玉东 刘秉乾 张雪培 魏金星 吴长利 |
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| 作者单位： | 1. 郑州大学第一附属医院泌尿外科,郑州,450052
2. 天津市泌尿外科研究所,天津,300211 |
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| 摘 要： | 目的:检测前列腺癌组织中骨桥蛋白(OPN)及基质金属蛋白酶-2(MMP-2)的表达。方法:对前列腺癌、良性前列腺增生与正常前列腺组织标本,应用半定量RT-PCR检测OPNmRNA的表达,应用免疫组织化学技术检测OPN和MMP-2蛋白的表达。结果:46例前列腺癌组织中OPNmRNA的表达阳性率为76.1%(35/46),OPN及MMP-2蛋白的表达阳性率分别为71.7%(33/46)和67.4%(31/46),两者表达呈正相关(P<0.01)。并且两者在前列腺癌不同分期和分级之间差异有统计学意义(P<0.05)。前列腺增生及正常前列腺组织中2者均未见阳性表达。结论:OPN阳性表达可能与肿瘤的浸润转移有关,OPN与MMP-2可能在前列腺癌的进展中起协同作用。
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| 关 键 词： | 前列腺肿瘤 癌 骨桥蛋白 基质金属蛋白酶-2 |
| 修稿时间： | 2004年12月28 |
Expression of osteopontin in prostate cancer and its correlation with matrix metalloproteinase-2 |
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| Wu Yudong,LIU Bingqian,ZHANG Xuepei,WEI Jinxing,WU Changli.Expression of osteopontin in prostate cancer and its correlation with matrix metalloproteinase-2[J].Journal of Zhengzhou University: Med Sci,2005,40(4):654-656. |
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| Authors: | Wu Yudong LIU Bingqian ZHANG Xuepei WEI Jinxing WU Changli |
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| Institution: | WU Yudong 1),LIU Bingqian 2),ZHANG Xuepei 1),WEI Jinxing 1),WU Changli 2) 1)Department of Urology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 4500522)Tianjin Institute of Urological Surgery,Tianjin 300211 |
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| Abstract: | Aim: To investigate the expression of osteopontin(OPN) in prostate cancer and its relationship with matrix metalloproteinase-2(MMP-2). Methods: The expression of OPN mRNA was detected using semi-quantitative RT-PCR,and the expression of OPN and MMP-2 protein,by S-P imunohistochemical assay. Results: The positive expression rates of OPN mRNA was 76.1%(35/46). The positive expression rates of OPN and MMP-2 were 71.7% (33/46) and 67.4% (31/46) in prostate cancer tissue, respectively, and the expression of OPN was significantly correlated with MMP-2 expression (P< 0.01 ). The expressions of OPN and MMP-2 were related to the tumor grade and clinical stage (P<0.05). Conclusion: OPN may play an important role in the progression of prostate cancer and is a potential marker for invasion and metastasis of prostate cancer. OPN and MMP-2 might play synergetic roles in prostate carcinogenesis. |
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| Keywords: | prostatic neoplasm carcinoma osteopontin matrix metalloproteinase-2 |
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