蚕丝水解物对实验性糖尿病大鼠血糖调节作用的机制研究

从胰岛素的受体和受体后作用机制来探讨蚕丝水解物（ｓｉｌｋ ｈｙｄｒａｔｅｓ，ＳＨ）调节糖代谢的机理。选用若干只雄性成年Ｗｉｓｔａｒ大鼠随机分为２组：一组经尾静脉注射小剂量链脲佐菌素（ＳＴＺ，３０ｍｇ／ｋｇＢＷ）３周后，加喂高糖、高脂、高热量饲料。选择具有Ⅱ型糖尿病特征，即糖耐量异常、胰岛素高于或等于对照组的３８只大鼠为糖尿病模型组。另一组３６只大鼠不注射ＳＴＺ，公喂普通饲料，为对照组。再分别将２组

Mechanism of silk hydrates on modulating the blood glucose metabolism in rats with experimental diabetes

Thirty eight adult male Wistar rats injected with streptozotocin(STZ) 30 mg/kg BW via tail vein and fed on a diet of high fat, high sucrose and high calorie for 3 weeks and then for another 8 weeks were successfully established as the diabetes model(type II diabetes mellitus). The effects of feeding silk hydrates(SH) for 12 weeks on the activities of insulin receptors and postreceptors were observed. The results showed that: 1) SH greatly improved the glucose tolerance of diabetic rats, but no effect on the secretion of insulin in rats with or without diabetes; 2) Only the low-affinity constant of insulin binding rates(K2) in skeletal muscle cell membranes of diabetic rats fed with SH were obviously increased, the high-affinity binding constant and the number of receptors were not changed; 3) the uptake of glucose in skeletal muscle of diabetic rats fed on SH was significantly increased. 4) SH partly improved the metabolism of serum lipids. The results indicated that SH could modulate the metabolism of blood glucose mainly through the mechanism on the activity of insulin postreceptor.