细胞块切片及免疫组化在恶性浆膜腔积液诊断中的应用体会

目的探讨运用细胞块切片结合免疫组化方法鉴别诊断腺癌与增生间皮细胞。方法对44例经证实的恶性浆膜腔积液患者，采用细胞学涂片与细胞块切片结合CEA、CK7、WT-1、Calretinin（CR）免疫组化检测研究。结果细胞块切片结合免疫组化的检出率明显优于常规细胞学涂片（P〈0．01）；CEA、CK7、CR是鉴别诊断腺癌细胞与增生间皮细胞较为有用的抗体。结论细胞块切片结合免疫组化是鉴别腺癌细胞与增生间皮细胞的有效方法，CEA、CK7、CR的抗体组合具有较高的临床应用价值。

Application of Cell Block Slice and Immunohistochemistry in Malignant Serous Cavity Effusion

Objective To discuss on the use of cell block slice and immunohistochemisty in differential diagnosis of adenocarcinoma and mesothelial proliferative cells. Methods Malignant serous cavity effusion of 44 cases were confirmed, and cytological smears, cell block slice with CEA, CK7, WT-1, Calretinin （CR） immunohistochemical detection were used. Results Detection rate of Cell block slice plus immunohistochemical method was superior to conventional cytological smear （ P 〈 0.01 ） ; CEA, CK7, CR were the useful types of antibodies for the differential diagnosis of adenocarcinoma and mesothelial proliferative cells. Conclusion Celt block slice combined with immunohistochemistry is the effective method for identifing adenocarcinoma and mesothelial proliferative cell. Antibody combinations of CEA, CK7, CR have a higher clinical application value.