| HCV RNA及其编码蛋白在肝癌和癌旁肝组织中的表达 |
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| 引用本文： | 寿芝萍,戴益民. HCV RNA及其编码蛋白在肝癌和癌旁肝组织中的表达[J]. 中华消化杂志, 1995, 15(4): 191-193 |
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| 作者姓名： | 寿芝萍 戴益民 |
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| 作者单位： | 第二军医大学病理学教研室，江苏启东市肝癌防治研究所 |
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| 摘 要： | 本文采用光敏生物素标记的ＨＣＶｃＤＮＡ探针和免疫金银显色法对４２例肝癌和癌旁肝组织中的ＨＣＶＲＮＡ和ＨＣＡｇ进行了检测，发现ＨＣＶＲＮＡ可以感染肝癌细胞，其分布状态及其抗原表达亦与肝组织一致。这些阳性细胞在组织中的感染状态多呈区域性，在此区域又可呈片状、簇状或散在分布。
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| 关 键 词： | 丙型肝炎病毒 肝细胞癌 原位杂交 免疫组织化学 |
EXPRESSION OF HEPATITIS C VIRUS RNA AND HCAg IN HUMAN HEPATOCELLULAR CARCINOMA AND ITS SURROUNDINGLIVER TISSUES |
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| Shou Zhiping,Dai Yiming,Ni Canrong,et al.. EXPRESSION OF HEPATITIS C VIRUS RNA AND HCAg IN HUMAN HEPATOCELLULAR CARCINOMA AND ITS SURROUNDINGLIVER TISSUES[J]. Chinese Journal of Digestion, 1995, 15(4): 191-193 |
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| Authors: | Shou Zhiping Dai Yiming Ni Canrong et al. |
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| Affiliation: | Shou Zhiping,Dai Yiming,Ni Canrong,et al. Department of Pathology,the Second Military Medical University. |
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| Abstract: | he expressions of hepatitis C virus(HCV)RNA and HCAg were investigated in 42 caseswith hepatocellular carcinoma and its surrounding liver tissues with non-radioactive in situhybridization and immunohistochemical method.The results showed that there are threetypes of HCV RNA hybridization signals,i.e., in the cytoplasm, nucleus,or both cytoplasmand nucleus;HCAg molecules were found in l0 out of 42 cases and expressed as bothcytoplasmic and inclusion types. HCV- infected cells(including cancer cells and hepatocytes)appeared diffuse,clustered or discrete in cancer and its surrounding liver tissue.We found that the liver cancer cells may be infected by HCV RNA just like normalhepatocytes and their distribution and expressing pattern of HCV RNA and HCAg aresimilar.With application of several probes to the ISH,positive signals of HCV RNA are morefrequent than using single probe. |
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| Keywords: | Hepatitis C virus Hepatocellular carcinoma In situ hybridization Immunohistochemistry. |
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